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PXD006304-1

PXD006304 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAnalysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
DescriptionWe interrogated whether there existed a broader effect of PINK1-dependent phosphorylation after mitochondrial damage by utilizing primary cultured neurons from WT and Pink1 KO mice. Because persistent depolarization leads to mitochondrial damage {}, we treated cultured neurons with the protonophore carbonyl cyanide m-chlorophenyl hydrazine (CCCP) and blotted for PINK1. Consistent with previous studies, CCCP treatment results in an upregulation of endogenous PINK1 in WT neurons. Endogenous PINK1 is undetectable in Pink1 KO neurons with CCCP treatment.Overall, our phosphoproteome analysis of biological duplicates experiments quantified 10930 and 7207 phosphorylation sites, respectively, from the early (designated as 15-45 min) and late (designated as 2-6 hr) treatment time points
HostingRepositoryMassIVE
AnnounceDate2017-04-14
AnnouncementXMLSubmission_2017-04-14_07:20:50.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterwanda
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListPhospho
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-04-14 05:43:43ID requested
12017-04-14 07:20:50announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PINK1 phosphoproteome apoptosis BAD
Contact List
Lujian Liao
contact affiliationShanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University
contact emailljliao@bio.ecnu.edu.cn
lab head
wanda
contact affiliationECNU
contact email782899013@qq.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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