PXD006304-1
PXD006304 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death |
| Description | We interrogated whether there existed a broader effect of PINK1-dependent phosphorylation after mitochondrial damage by utilizing primary cultured neurons from WT and Pink1 KO mice. Because persistent depolarization leads to mitochondrial damage {}, we treated cultured neurons with the protonophore carbonyl cyanide m-chlorophenyl hydrazine (CCCP) and blotted for PINK1. Consistent with previous studies, CCCP treatment results in an upregulation of endogenous PINK1 in WT neurons. Endogenous PINK1 is undetectable in Pink1 KO neurons with CCCP treatment.Overall, our phosphoproteome analysis of biological duplicates experiments quantified 10930 and 7207 phosphorylation sites, respectively, from the early (designated as 15-45 min) and late (designated as 2-6 hr) treatment time points |
| HostingRepository | MassIVE |
| AnnounceDate | 2017-04-14 |
| AnnouncementXML | Submission_2017-04-14_07:20:50.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | wanda |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Phospho |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-04-14 05:43:43 | ID requested | |
| ⏵ 1 | 2017-04-14 07:20:50 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: PINK1 phosphoproteome apoptosis BAD |
Contact List
| Lujian Liao | |
|---|---|
| contact affiliation | Shanghai Key Laboratory of Regulatory Biology, School of Life Sciences, East China Normal University |
| contact email | ljliao@bio.ecnu.edu.cn |
| lab head | |
| wanda | |
| contact affiliation | ECNU |
| contact email | 782899013@qq.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080923 |
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