PXD006262-1

PXD006262 is an original dataset announced via ProteomeXchange.

Title	S. cerevisiae proteome response to heat stress - 240 min time course.
Description	In this project, we follow the temporal proteome dynamics during heat stress response in a model organism Saccharomyces cerevisiae. Using label-free quantification we determine changes in the yeast proteome at different times after mild temperature shift, from 30˚C to 37˚C. The time points measured were 0 min (30˚C, no stress) and then at 10 min, 30 min, 60 min, 120 min and 240 min after cells were transferred to 37 °C. For each time point, four biological replicates were collected for the haploid wild type (BY4742, Mat ALPHA, his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; YJL088w::kanMX4) strain and a haploid strain with a chaperone Ssb1p deletion (ssb1Δ).
HostingRepository	PRIDE
AnnounceDate	2018-01-17
AnnouncementXML	Submission_2018-01-17_11:27:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Jarnuczak
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-05 02:48:53	ID requested
⏵ 1	2018-01-17 11:27:58	announced
2	2018-01-17 11:36:33	announced	Updated publication reference for DOI(s): 10.1039/C7MO00050B.
3	2018-02-24 00:31:11	announced	Updated project metadata.
4	2023-11-14 08:49:16	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: environmental stress response, label free quantification, HSP70 chaperone

Professor Simon Hubbard
contact affiliation	Division of Evolution and Genomic Sciences School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Oxford Road, Manchester M13 9PT
contact email	simon.hubbard@manchester.ac.uk
lab head
Andrew Jarnuczak
contact affiliation	EBI
contact email	jarnuczak@ebi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006262

PRIDE project URI

• PRIDE
  1. PXD006262
     1. Label: PRIDE project
     2. Name: S. cerevisiae proteome response to heat stress - 240 min time course.