PXD006230-1

PXD006230 is an original dataset announced via ProteomeXchange.

Title	Proteomics and Phosphoproteomics of an Inducible HIV
Description	The mechanisms by which human immunodeficiency virus (HIV) circumvents cellular defense machinery to replicate and persist in cells are not fully understood. HIV accessory proteins play key roles in the HIV life cycle by altering host signaling pathways that are highly dependent on post-translational modification (PTM) events. Thus, the identification of HIV accessory protein host targets and their PTM status is critical to fully understand how HIV invades, avoids detection and replicates to spread infection. To date, a comprehensive characterization of HIV accessory protein host targets and modulation of their PTM status does not exist. The significant gap in knowledge regarding the identity and PTMs of HIV host targets is due, in part, to technological limitations. Through this study we sought to apply current mass spectrometry techniques to define mechanisms of viral protein action through identifying host protein interaction partners of Vpr and modulation of down-stream PTM based signaling pathways. Here we report identification and abundance dynamics of over 7,000 proteins and 28,000 phospho-peptides. By utilizing a novel, inducible HIV-1 CD4+ T-cell model system, we overcame challenges associated with synchronization and infection present in other models. This inducible HIV-1 model, in conjunction with state of the art quantitative multiplexed proteomics, allowed for accurate assessment of early protein and PTM dynamics associated with induction of HIV expression. This thorough characterization of Vpr interactions and PTM temporal dynamics, assessed by comparing the wild type with a Vpr deficient strain, deepens the understanding of how HIV circumvents immunity to hijack host cells. This study acts as a resource that lays the foundation for validating host proteins and important PTM based signaling pathways as viable intervention targets.
HostingRepository	MassIVE
AnnounceDate	2017-06-13
AnnouncementXML	Submission_2017-06-13_07:21:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Lapek
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: HIV-1 unknown group; NCBI TaxID: 540993;
ModificationList	Phospho; Oxidation; Carbamidomethyl; TMT6plex
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-01 16:08:02	ID requested
⏵ 1	2017-06-13 07:21:39	announced

John D. Lapek, Jr., Mary K Lewinski, Jacob M Wozniak, John Guatelli, and David J Gonzalez. Quantitative temporal viromics of an inducible HIV-1 model yields insight to global host targets and phospho-dynamics associated with Vpr. Mol Cell Proteomics mcp.M116.066019. First Published on June 12, 2017, doi:10.1074/mcp.M116.066019.

submitter keyword: HIV, Jurkat, TMT, Proteomics

David Gonzalez
contact affiliation	UCSD
contact email	djgonzalez@ucsd.edu
lab head
John Lapek
contact affiliation	UCSD
contact email	jlapek@ucsd.edu
dataset submitter

MassIVE dataset URI

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