PXD006229-1

PXD006229 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency
Description	Chromatin-associated fumarase (FH) affects histone methylation via its metabolic activity. However, whether this regulation is implicated in gene transcription remains to be clarified. In this study, we show that glucose deprivation induces AMPK-dependent phosphorylation of FH at Ser 75, which promotes association of FH with transcriptional factor ATF2 and related gene promoters. The inhibitory effect of local fumarate on KDM2A histone demethylase maintains H3K36me2, thus facilitates transcription for cell growth arrest. On the other hand, FH is found to be O-GlcNAcylated at the AMPK phosphorylation site; FH-ATF2-mediated downstream events are impeded by FH O-GlcNAcylation especially in cancer cells that display the robust OGT activity. Consistently, FH-S75 phosphorylation levels inversely correlate with the OGT level and poor prognosis in pancreatic cancer patients. These findings uncover a mechanism underlying transcription regulation by metabolic enzyme and the linkage between dysregulated OGT activity and growth advantage of cancer cells under nutrient stress.
HostingRepository	MassIVE
AnnounceDate	2017-04-01
AnnouncementXML	Submission_2017-04-01_04:28:42.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Houqin
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Phospho; O-(N-acetylamino)glucosyl-L-serine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-04-01 03:58:10	ID requested
⏵ 1	2017-04-01 04:28:42	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: fumarase, phosphorylation, O-GlcNAcylation

submitter keyword: fumarase, phosphorylation, O-GlcNAcylation

Contact List

Yuhui Jiang
contact affiliation	The Institute of Cell Metabolism, Shanghai Key Laboratory of Pancreatic Disease, Shanghai General Hospital, Shanghai Jiaotong University, School of Medicine
contact email	oxfordman@hotmail.com
lab head
Houqin
contact affiliation	ECNU
contact email	1160663589@qq.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080872