PXD006224-1

PXD006224 is an original dataset announced via ProteomeXchange.

Title	pig GIT meatproteome
Description	Background: The possible impact of changes in diet composition for the intestinal microbiome is mostly studied after some days of adaptation to the diet of interest. The question arises if few days are enough to reflect the microbial response to the diet by changing the community composition and function. The present study investigated the fecal microbiome of pigs in a time span of four weeks after a dietary change to get an insight of the needed adaptation period. Four different diets were used differing in either protein source (field peas meal vs. soybean meal) or the concentration of calcium and phosphorus (CaP). Results: Twelve pigs were sampled at seven time points within four weeks after the dietary change. Fecal samples were used to sequence the 16S rDNA amplicons, to analyse the microbial proteins via LC-MS/MS and to determine the SCFA production. The analysis of OTU abundances and quantification values of proteins showed a significant separation of three periods of time (p=0.001). Samples from the first day are used to define the ‘Zero phase’, samples of weeks one and two are combined as ‘metabolic phase’ and an ‘equilibrium phase’ was defined based on samples from week three and four. Only in this last phase, a separation according to the supplementation of CaP was significantly detectable (p=0.001). No changes were found based on the corn-soybean meal or corn-field peas administration. The analysis of possible factors causing this significant separation showed only an overall change of bacterial members and functional properties. The metaproteomic approach yields a total of about 9700 proteins, which were used to deduce possible metabolic functions of the bacterialcommunity.
HostingRepository	PRIDE
AnnounceDate	2017-10-26
AnnouncementXML	Submission_2017-10-26_05:11:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Seifert
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Bacteria; NCBI TaxID: 2;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-31 00:46:54	ID requested
⏵ 1	2017-10-26 05:11:35	announced
2	2017-11-01 08:52:11	announced	Updated publication reference for PubMed record(s): 29078812.
3	2024-10-22 04:01:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: porcine GIT, metaproteome, bacteria, Sus scrofa, LC/MSMS

Jana Seifert
contact affiliation	University of Hohenheim, Institute of Animal Science, Stuttgart, Germany
contact email	jseifert@uni-hohenheim.de
lab head
Jana Seifert
contact affiliation	University of Hohenheim
contact email	jseifert@uni-hohenheim.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006224

PRIDE project URI

• PRIDE
  1. PXD006224
     1. Label: PRIDE project
     2. Name: pig GIT meatproteome