PXD006196-2

PXD006196 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	moDCs HPLC-MS/MS
Description	To investigate the allergenicity of the major birch pollen allergen Bet v 1 and the impact of known adjuvants coming from pollen, such as lipopolysaccharide (LPS), we performed quantitative proteome analysis of stimulated monocyte-derived dendritic cells (moDCs). Thus, we treated cells with birch pollen extract (BPE), a recombinant variant of Bet v 1 or LPS followed by proteomic profiling by means of high performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) using isobaric labelling.
HostingRepository	PRIDE
AnnounceDate	2017-12-01
AnnouncementXML	Submission_2017-12-11_04:05:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lisa Strasser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-29 01:05:07	ID requested
1	2017-11-30 18:42:07	announced
⏵ 2	2017-12-11 04:05:22	announced	Updated publication reference for PubMed record(s): 29192156.
3	2024-10-22 04:05:18	announced	2024-10-22: Updated project metadata.

Publication List

Strasser L, Dang HH, Schwarz H, Asam C, Ferreira F, Horejs-Hoeck J, Huber CG, Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells. Sci Rep, 7(1):16638(2017) [pubmed]

Strasser L, Dang HH, Schwarz H, Asam C, Ferreira F, Horejs-Hoeck J, Huber CG, Unbiased Quantitative Proteomics Reveals a Crucial Role of the Allergen Context for the Activation of Human Dendritic Cells. Sci Rep, 7(1):16638(2017) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: Human, moDC, HPLC-MS/MS

curator keyword: Biomedical

submitter keyword: Human, moDC, HPLC-MS/MS

Contact List

Christian G. Huber
contact affiliation	University of Salzburg, Department of Molecular Biology, Division of Chemistry and Bioanalytics, Hellbrunnerstraße 34, 5020 Salzburg, Austria
contact email	c.huber@sbg.ac.at
lab head
Lisa Strasser
contact affiliation	Universität Salzburg
contact email	lisa.strasser@sbg.ac.at
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006196

PRIDE project URI

Repository Record List

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