PXD006177-1

PXD006177 is an original dataset announced via ProteomeXchange.

Title	Stable isotope-labeling-based quantitative proteomic analysis of ethylene-regulated acetylome in Arabidopsis
Description	Acetylation has been demonstrated to be a global post-translational modification. However, identification of acetylation sites in Arabidopsis is insufficient and its ethylene-induced changes in acetylome remains unknown. Here we use a robust quantitative proteomics strategy which is dimethyl-label based extracted ion chromatogram quantitation to monitor the acetylome changes after long-term ethylene treatment in Arabidopsis EIN3 (Ethylene Insensitive 3)/EIL1 (Ethylene Insensitive 3-Like 1) double mutants. In total, 6,431 acetylation sites were identified from 3,039 proteins. Among them, 30 ethylene-enhanced and 27 ethylene-suppressed acetyl-peptides were quantified from various proteins, including histone proteins, ribosomal proteins and heat shock proteins. Long-term ethylene treatment led to smaller rosette diameters and shorter root lengths in both wild type and ein3/eil1 mutants of Arabidopsis, which indicates that ethylene can regulate plant growth and development by both EIN3/EIL1-dependent and –independent ways. According to the quantitation results, these regulation may happen during the processes of gene transcription, protein translation and protein folding.
HostingRepository	PRIDE
AnnounceDate	2018-02-21
AnnouncementXML	Submission_2018-02-21_01:53:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fengchao Yu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	2x(13)C: 4x(2)H labeled dimethylated residue; monohydroxylated residue; acetylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-27 03:28:50	ID requested
⏵ 1	2018-02-21 01:53:17	announced
2	2018-03-16 01:30:16	announced	Updated project metadata.
3	2024-10-22 04:05:13	announced	2024-10-22: Updated project metadata.

Liu S, Yu F, Yang Z, Wang T, Xiong H, Chang C, Yu W, Li N, Establishment of Dimethyl Labeling-based Quantitative Acetylproteomics in Arabidopsis. Mol Cell Proteomics, 17(5):1010-1027(2018) [pubmed]

curator keyword: Biological

submitter keyword: Hormone response, stable isotope dimethyl labeling, acetylome, quantitative PTM proteomics, plant growth and development, cell signaling

Ning Li
contact affiliation	Division of Life Science, The Hong Kong University of Science and Technology
contact email	boningli@ust.hk
lab head
Fengchao Yu
contact affiliation	The Hong Kong University of Science and Technology
contact email	fyuab@connect.ust.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006177
     1. Label: PRIDE project
     2. Name: Stable isotope-labeling-based quantitative proteomic analysis of ethylene-regulated acetylome in Arabidopsis