PXD006171-1

PXD006171 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome and proteome of T. cruzi metacyclogenesis
Description	Trypanosoma cruzi metacyclogenesis is a natural process that occurs inside triatomine insect vector and corresponds to the differentiation of non-infective epimastigotes into infective metacyclic trypomastigotes. Here, centered on LC-MS/MS approach, a large scale phospho (proteome) study was carried out aiming to quantitatively understand the metacyclogenesis. Cells were colected along metacyclogenesis totalizing six points, stress 2 hours, adhered 12, 24 and 48 hours, and metacyclic trypomastigote from two biological replicates. After extraction, total protein were digested, fractioned and analyzed by LC-MS/MS. The samples for phosphoproteomics were processed for phosphopeptide enrichment prior to the analysis.
HostingRepository	PRIDE
AnnounceDate	2017-08-31
AnnouncementXML	Submission_2017-08-31_09:19:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	juliana carolina amorim
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: 1416333;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-27 01:32:00	ID requested
⏵ 1	2017-08-31 09:19:32	announced
2	2024-10-22 04:05:06	announced	2024-10-22: Updated project metadata.

Amorim JC, Batista M, da Cunha ES, Lucena ACR, Lima CVP, Sousa K, Krieger MA, Marchini FK, Quantitative proteome and phosphoproteome analyses highlight the adherent population during Trypanosoma cruzi metacyclogenesis. Sci Rep, 7(1):9899(2017) [pubmed]

curator keyword: Biological

submitter keyword: Trypanosoma cruzi, metacyclogenesis, phosphorylation, proteomics

Fabricio Klerynton Marchini
contact affiliation	Funcional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil
contact email	fabricio.marchini@fiocruz.br
lab head
juliana carolina amorim
contact affiliation	Carlos Chagas Institute
contact email	julianacarolinaamorim@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006171

PRIDE project URI

• PRIDE
  1. PXD006171
     1. Label: PRIDE project
     2. Name: Phosphoproteome and proteome of T. cruzi metacyclogenesis