PXD006156 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling |
Description | RNF8 is a key E3 ubiquitin ligase functioning in both DSB- and UV-induced DDR, identification of additional substrates will help further elucidate its role in DNA damage signaling. To identify substrates of ubiquitin ligases, we have recently devised a method based on proximity-dependent biotin labeling. In this method, an E3 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase BirA together with a biotin acceptor peptide (AP)-tagged ubiquitin. The BirA-directed biotin labeling of AP depends on the proximity of the two fusion proteins in the cell, which leads to preferential labeling of ubiquitinated E3 substrates. In this study, we applied this procedure to RNF8 and identified its substrates. |
HostingRepository | PRIDE |
AnnounceDate | 2018-11-07 |
AnnouncementXML | Submission_2019-02-12_08:18:40.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD006156 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rakesh Deshar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl; GlyGly |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-22 06:21:21 | ID requested | |
1 | 2018-11-07 01:21:51 | announced | |
⏵ 2 | 2019-02-12 08:18:42 | announced | Updated publication reference for PubMed record(s): 30445466. |
Publication List
Deshar R, Yoo W, Cho EB, Kim S, Yoon JB, RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling. Nucleic Acids Res, 47(2):762-778(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: RNF8, E3 ligase, substrate identification, DNA damage |
Contact List
Jong-Bok Yoon |
contact affiliation | Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University, 262 Seongsanno, Seodaemun-Gu, Seoul 120-749, Korea. Tel.: +82-2-2123-2704; Fax: 82-2-362- 9897 |
contact email | yoonj@yonsei.ac.kr |
lab head | |
Rakesh Deshar |
contact affiliation | The Catholic University of Korea |
contact email | rakeshdesar@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006156
- Label: PRIDE project
- Name: RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling