PXD006148 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Discovery of Mouse Spleen Signaling Responses to Anthrax using Label-Free Quantitative Phosphoproteomics via Mass Spectrometry |
Description | From article abstract: Inhalational anthrax is caused by spores of the bacterium Bacillus anthracis (B. anthracis), and is an extremely dangerous disease that can kill unvaccinated victims within 2 weeks. Modern antibiotic-based therapy can increase the survival rate to ~50%, but only if administered presymptomatically (within 24-48 h of exposure). To discover host signaling responses to presymptomatic anthrax, label-free quantitative phosphoproteomics via liquid chromatography coupled to mass spectrometry was used to compare spleens from uninfected and spore-challenged mice over a 72 h time-course. Late-stage (48-72 h post-challenge) Sterne strain (lethal) infections resulted in global alterations to the spleen phosphoproteome. In contrast, delta-Sterne strain (asymptomatic; missing the anthrax toxin) infections resulted in 188 (5.8%) significantly altered (q<0.05) phosphopeptides. Twenty-six highly tentative phosphorylation responses to early-stage (24 h post-challenge) anthrax were discovered (q<0.5), and ten of these originated from eight proteins that have known roles in the host immune response. These tentative early-anthrax host response signaling events within mouse spleens may translate into presymptomatic diagnostic biomarkers of human anthrax detectable within circulating immune cells, and could aid in the identification of pathogenic mechanisms and therapeutic targets. [Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.000927, 1–11, 2011. The files in this dataset are exactly the same files that were originally uploaded to Proteome Commons Tranche as described in the article.] |
HostingRepository | PRIDE |
AnnounceDate | 2017-03-22 |
AnnouncementXML | Submission_2017-03-22_05:25:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nathan Manes |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bacillus anthracis str. Sterne; NCBI TaxID: 260799; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-21 09:10:07 | ID requested | |
⏵ 1 | 2017-03-22 05:25:06 | announced | |
Publication List
Manes NP, Dong L, Zhou W, Du X, Reghu N, Kool AC, Choi D, Bailey CL, Petricoin EF, Liotta LA, Popov SG, Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics, 10(3):M110.000927(2011) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Mouse, Spleen, Anthrax, LC-MSMS, Label-Free, Phosphoproteomics |
Contact List
Serguei Popov |
contact affiliation | George Mason University, VA |
contact email | spopov@gmu.edu |
lab head | |
Nathan Manes |
contact affiliation | National Institutes of Health |
contact email | nathan.manes@nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006148
- Label: PRIDE project
- Name: Discovery of Mouse Spleen Signaling Responses to Anthrax using Label-Free Quantitative Phosphoproteomics via Mass Spectrometry