PXD006141-2

PXD006141 is an original dataset announced via ProteomeXchange.

Title	Exploring the potential role of moonlighting function of the surface-associated proteins from Mycobacterium bovis BCG Moreau and Pasteur by comparative proteomic
Description	Tuberculosis (TB) is one of major causes of death worldwide. Bacillus Calmette-Guerin (BCG) is the only licensed TB vaccine and its inability to protect against adult pulmonary TB can be due to genetic differences among strains described since the 1940s. In this work, we compared the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau, the Brazilian vaccine strain, and the BCG Pasteur reference strain. The methodology used was 2D-gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF). We identified 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits.
HostingRepository	PRIDE
AnnounceDate	2019-05-16
AnnouncementXML	Submission_2019-05-16_00:13:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dario E. Kalume
SpeciesList	scientific name: Mycobacterium bovis BCG str. Moreau RDJ; NCBI TaxID: 413996;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-21 04:49:21	ID requested
1	2019-05-15 00:58:19	announced
⏵ 2	2019-05-16 00:13:39	announced	Updated project metadata.
3	2024-10-22 04:04:37	announced	2024-10-22: Updated project metadata.

Pagani TD, Guimar, ã, es ACR, Waghabi MC, Corr, ê, a PR, Kalume DE, Berr, ê, do-Pinho M, Degrave WM, Mendon, ç, a-Lima L, BCG Moreau and Pasteur by Comparative Proteomic. Front Immunol, 10():716(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mycobacterium bovis BCG Moreau, moonlighting function, surface-associated proteins

Da¡rio Eluan Kalume
contact affiliation	Laboratorio Interdisciplinar de Pesquisas Medicas, LIPMED,Instituto Oswaldo Cruz,IOC,Fundacao Oswaldo Cruz, FIOCRUZ,Rio de Janeiro,Brazil
contact email	kalume1@yahoo.com
lab head
Dario E. Kalume
contact affiliation	FIOCRUZ
contact email	kalume1@yahoo.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006141
     1. Label: PRIDE project
     2. Name: Exploring the potential role of moonlighting function of the surface-associated proteins from Mycobacterium bovis BCG Moreau and Pasteur by comparative proteomic