PXD006140-1

PXD006140 is an original dataset announced via ProteomeXchange.

Title	Subproteome persulfidation reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis thaliana
Description	Hydrogen sulfide-mediated signaling pathways regulates many physiological and pathophysiological processes in mammalian and plant systems. The molecular mechanism by which hydrogen sulfide exerts its action involves the post-translational modification of cysteine residues to form a persulfidated thiol motif, named as protein S-sulfhydration or persulfidation. We have developed a comparative and quantitative proteomic analysis approach for the detection of endogenous S-sulfhydrated proteins in wild-type Arabidopsis and L-CYSTEINE DESULFHYDRASE 1 mutant leaves by using the tag-switch method. Bioinformatic analysis of the identified proteins revealed that S-sulfhydrated cysteines are part of a wide range of biological functions regulating important processes such as carbon metabolism, plant responses to abiotic and biotic stresses, plant growth and development, and RNA translation. Quantitative analysis in both genetic backgrounds reveals that protein sulfhydration is mainly involved in primary metabolic pathways such as tricarboxylic acid cycle, glycolysis or Calvin cycle, suggesting that this protein modification is a new regulatory component in these pathways
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_14:50:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Angeles Aroca
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-21 04:44:13	ID requested
⏵ 1	2018-10-24 14:50:08	announced
2	2024-10-22 04:04:40	announced	2024-10-22: Updated project metadata.

Aroca A, Benito JM, Gotor C, Romero LC, Persulfidation proteome reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis. J Exp Bot, 68(17):4915-4927(2017) [pubmed]

curator keyword: Biological

submitter keyword: Mass spectrometry, proteomic, cysteine, hydrogen sulfide, S-sulfhydration, post-translational modification

Luis Carlos Romero Gonzalez
contact affiliation	Instituto de Bioquimica Vegetal y Fotosintesis CSIC Americo Vespucio 49. 41092. Seville (Spain) +34954489506
contact email	lromero@ibvf.csic.es
lab head
Angeles Aroca
contact affiliation	IBVF-CSIC
contact email	angeles.aroca@ibvf.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006140

PRIDE project URI

• PRIDE
  1. PXD006140
     1. Label: PRIDE project
     2. Name: Subproteome persulfidation reveals the regulation of protein function by hydrogen sulfide in diverse biological processes in Arabidopsis thaliana