PXD006120-2

PXD006120 is an original dataset announced via ProteomeXchange.

Title	Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan
Description	Aging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. Drosophila melanogaster, we observed differential regulation of BM collagens between laboratory strains, correlating with changes in muscle physiology leading to cardiac dysfunction. Therefore, we sought to understand the extent to which BM proteins modulate contractile function during aging. Cardiac-restricted knockdown of ECM genes Pericardin, Laminin A, and Viking in Drosophila prevented age-associated heart tube restriction and increased contractility, even under viscous load. Most notably, reduction of Laminin A expression correlated with an overall preservation of contractile velocity with age and extension of organismal lifespan. Global heterozygous knockdown confirmed these data, which provides new evidence of a direct link between BM homeostasis, contractility, and maintenance of lifespan.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:42.765.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koen Raedschelders
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-20 04:36:36	ID requested
1	2017-03-27 01:40:24	announced
⏵ 2	2024-10-22 04:04:43	announced	2024-10-22: Updated project metadata.

Sessions AO, Kaushik G, Parker S, Raedschelders K, Bodmer R, Van Eyk JE, Engler AJ, Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan. Matrix Biol, 62():15-27(2017) [pubmed]

10.1016/j.matbio.2016.10.008;

curator keyword: Biological, Biomedical

submitter keyword: Basement membrane, Laminin A,Aging, Extracellular matrix, Drosophila

Jennifer Van Eyk
contact affiliation	Cedars-Sinai Medical Center, Heart Institute, Los Angeles, CA. United States of America
contact email	jennifer.vaneyk@cshs.org
lab head
Koen Raedschelders
contact affiliation	Cedars-Sinai Medical Center
contact email	koen.raedschelders@cshs.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD006120

PRIDE project URI

• PRIDE
  1. PXD006120
     1. Label: PRIDE project
     2. Name: Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan