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PXD006092-1

PXD006092 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStUbEx PLUS in U2OS .
DescriptionStUbEx PLUS – a modified Stable tagged Ubiquitin Exchange system for Peptide Level purification and in-depth mapping of Ubiquitination Sites. Applying StUbEx PLUS to U2OS cells treated with proteasomal inhibitors MG132 or Bortezomib resulted in the identification of 41,582 sites mapping on 7,762 proteins. In addition, 72 proteins were identified with N-terminal protein ubiquitination.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:06:32.684.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIñigo Barrio
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-14 07:02:36ID requested
12024-10-07 10:06:33announced
Publication List
10.1021/acs.jproteome.7b00566;
Akimov V, Olsen LCB, Hansen SVF, Barrio-Hernandez I, Puglia M, Jensen SS, Solov'yov IA, Kratchmarova I, Blagoev B, StUbEx PLUS-A Modified Stable Tagged Ubiquitin Exchange System for Peptide Level Purification and In-Depth Mapping of Ubiquitination Sites. J Proteome Res, 17(1):296-304(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: site specific ubiquitination
ubiquitination sites
N-terminal ubiquitination
proteasomal inhibition
MG132: Bortezomib
Contact List
Blagoy Blagoev
contact affiliationDepartment of Biochemistry and Molecular Biology, University of Southern Denmark (SDU)
contact emailbab@bmb.sdu.dk
lab head
Iñigo Barrio
contact affiliationSDU
contact emailinigob@bmb.sdu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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