PXD006078 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ovarian Cancer Lipid raft proteomics and role of OPCML |
Description | ipid rafts are dynamic membrane micro-domains that orchestrate molecular interactions and are implicated in cancer development. To understand potential role of tumor suppressor OPCML on the altered dynamics of lipid raft residing proteins, we performed quantitative (SILAC) proteomics experiment. We recently found that OPCML negatively regulates a subset of receptor tyrosine kinases (RTKs) by altering their recycling and ubiquitin-mediated degradation via sequestration to lipid rafts; however, the molecular mechanism linking OPCML tumor suppressor expression to altered RTK trafficking remains unclear. Here we performed a quantitative lipid raft proteomics study to dissect raft-mediated mechanism of OPCML-regulated tumor suppression. We also examined raft-associated mechanism of OPCML P95R, a common mutation resulting in substitution of proline to arginine at position 95, mediated regulation of adhesion potential of tumors. |
HostingRepository | PRIDE |
AnnounceDate | 2017-03-16 |
AnnouncementXML | Submission_2017-03-16_02:43:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anup Shah |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | 6520 Quadrupole Time-of-Flight LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-13 03:03:17 | ID requested | |
⏵ 1 | 2017-03-16 02:43:20 | announced | |
Publication List
Shah AD, Inder KL, Shah AK, Cristino AS, McKie AB, Gabra H, Davis MJ, Hill MM, Integrative Analysis of Subcellular Quantitative Proteomics Studies Reveals Functional Cytoskeleton Membrane-Lipid Raft Interactions in Cancer. J Proteome Res, 15(10):3451-3462(2016) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: OPCML, Lipid raft, Skov-3, SILAC, P95R OPCML |
Contact List
Michelle M. Hill |
contact affiliation | Associate Professor, Head Cancer Proteomics Group, University of Queensland Diamantina Institute, Woolloongabba, QLD, 4102 |
contact email | m.hill2@uq.edu.au |
lab head | |
Anup Shah |
contact affiliation | University of Queensland |
contact email | a.shah3@uq.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006078
- Label: PRIDE project
- Name: Ovarian Cancer Lipid raft proteomics and role of OPCML