PXD006063-1

PXD006063 is an original dataset announced via ProteomeXchange.

Title	Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 4
Description	Chinese Hamster Ovary (CHO) cells are frequently used to produce recombinant HIV envelope protein gp120. In this study, we characterized the CHO proteins that become co-purified with gp120 when lectin affinity chromatography is used. Many of co-purified CHO proteins identified were extracellular matrix components. We verified a method for separating gp120 from these CHO proteins with anion exchange chromatography, and assessed the biochemical activity of gp120 preparations with and without co-purified CHO proteins.
HostingRepository	PRIDE
AnnounceDate	2017-09-04
AnnouncementXML	Submission_2017-09-04_00:05:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Plotnik
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-09 01:37:19	ID requested
⏵ 1	2017-09-04 00:05:09	announced
2	2024-10-22 04:08:56	announced	2024-10-22: Updated project metadata.

Plotnik D, Guo W, Cleveland B, von Haller P, Eng JK, Guttman M, Lee KK, Arthos J, Hu SL, . J Virol, 91(21):(2017) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: HIV, gp120, fibronectin, extracellular matrix, CHO, Chinese Hamster

Shiu-Lok Hu
contact affiliation	Unversity of Washington
contact email	hus@wanprc.org
lab head
David Plotnik
contact affiliation	University of Washington
contact email	dplotnik@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006063
     1. Label: PRIDE project
     2. Name: Chinese Hamster Ovary cell proteins co-purified with recombinant HIV-1 envelopes, part 4