PXD006046 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | KCC2 Proteome |
Description | KCC2 is a neuron-specific K+-Cl‑ cotransporter essential for establishing the Cl- gradient required for hyperpolarizing inhibition. KCC2 is highly localized to excitatory synapses where it regulates spine morphogenesis and AMPA receptor confinement. Aberrant KCC2 function contributes to numerous human neurological disorders including epilepsy and neuropathic pain. Using unbiased functional proteomics, we identified the KCC2-interactome with the goal of determining KCC2-protein interactions that regulate KCC2 function. Our analysis revealed that KCC2 interacts with a diverse set of proteins, and its most predominant interactors play important roles in postsynaptic receptor recycling. The most abundant KCC2 interactor is a neuronal endocytic regulatory protein termed PACSIN1 (SYNDAPIN1). We verified the PACSIN1-KCC2 interaction biochemically and demonstrated that shRNA knockdown of PACSIN1 in hippocampal neurons significantly increases KCC2 expression and hyperpolarizes the reversal potential for Cl-. Overall, our global native-KCC2 interactome and subsequent characterization revealed PACSIN1 as a novel and potent negative regulator of KCC2. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-16 |
AnnouncementXML | Submission_2017-10-16_09:01:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vivek Mahadevan |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | (R)-5-oxo-1: 4-tetrahydrothiazine-3-carboxylic acid; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-03-07 08:20:41 | ID requested | |
⏵ 1 | 2017-10-16 09:01:56 | announced | |
2 | 2017-11-06 06:31:05 | announced | Updated publication reference for PubMed record(s): 29028184. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Neuronal Chloride homeostasis, Cation chloride cotransporter, KCC2, SLC12A5, LC/MS, Proteomics, Interacting proteins, GABA, Glutamate, E/I balance |
Contact List
Melanie Woodin |
contact affiliation | Associate Professor, Department of Cell and Systems Biology Director, Human Biology Program University of Toronto, Canada |
contact email | m.woodin@utoronto.ca |
lab head | |
Vivek Mahadevan |
contact affiliation | University of Toronto |
contact email | vivek.mahadevan@mail.utoronto.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006046 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006046
- Label: PRIDE project
- Name: KCC2 Proteome