PXD006039-1

PXD006039 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium tuberculosis under nitric oxide stress
Description	To study the entire transcriptional and translational M. tuberculosis response from initial survival to eventual escape from nitric oxide (NO) stress, we exposed exponentially growing M. tuberculosis to 1 mM diethylenetriamine/nitric oxide (DETA/NO) and followed the adaptive response over 48 hours. Samples were obtained from two independent experiments performed in triplicate and we sampled aliquots for transcriptome profiling by RNA sequencing at 20 min, 2 h and 24 h and for mass spectrometry-based shotgun proteomics at 20 min, 40 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h post NO exposure.
HostingRepository	PRIDE
AnnounceDate	2017-08-17
AnnouncementXML	Submission_2017-08-16_23:49:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olga Schubert
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-07 01:37:05	ID requested
⏵ 1	2017-08-16 23:49:02	announced
2	2024-10-22 04:07:20	announced	2024-10-22: Updated project metadata.

Cortes T, Schubert OT, Banaei-Esfahani A, Collins BC, Aebersold R, Young DB, Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival. Sci Rep, 7(1):8208(2017) [pubmed]

curator keyword: Biological

submitter keyword: Mycobacterium tuberculosis, shotgun proteomics, data-dependent acquisition, Orbitrap

Ruedi Aebersold
contact affiliation	Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Switzerland
contact email	aebersold@imsb.biol.ethz.ch
lab head
Olga Schubert
contact affiliation	University of California, Los Angeles
contact email	olga.schubert@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006039

PRIDE project URI

• PRIDE
  1. PXD006039
     1. Label: PRIDE project
     2. Name: Mycobacterium tuberculosis under nitric oxide stress