PXD006036-2

PXD006036 is an original dataset announced via ProteomeXchange.

Title	Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis
Description	A hallmark of cancer cells is the metabolic switch from oxidative phosphorylation (OXPHOS) to glycolysis, a phenomenon referred to as the “Warburg effect”, which is also observed in primed human pluripotent stem cells (hPSCs). Here, we report that downregulation of SIRT2 and upregulation of SIRT1 is a molecular signature of primed hPSCs and that SIRT2 critically regulates metabolic reprogramming during induced pluripotency by targeting glycolytic enzymes including aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and enolase. Remarkably, knockdown of SIRT2 in human fibroblasts resulted in significantly decreased OXPHOS and increased glycolysis. In addition, we found that miR-200c-5p specifically targets SIRT2, downregulating its expression. Furthermore, SIRT2 overexpression in hPSCs significantly affected energy metabolism, altering stem cell functions such as pluripotent differentiation properties. Taken together, our results identify the miR-200c-SIRT2 axis as a key regulator of metabolic reprogramming (Warburg-like effect), via regulation of glycolytic enzymes, during human induced pluripotency and pluripotent stem cell function. To address our hypothesis that acetylation affects the metabolic switch, we compared protein acetylation in hESCs and hDFs by liquid chromatography-tandem mass spectrometry (LCMS/ MS) analyses following immunoprecipitation with acetyl-Lys antibody.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:18.971.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006036
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Byung-gyu kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-06 06:15:49	ID requested
1	2018-10-26 09:17:45	announced
⏵ 2	2024-10-22 04:07:19	announced	2024-10-22: Updated project metadata.

10.1038/ncb3517;

Cha Y, Han MJ, Cha HJ, Zoldan J, Burkart A, Jung JH, Jang Y, Kim CH, Jeong HC, Kim BG, Langer R, Kahn CR, Guarente L, Kim KS, Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis. Nat Cell Biol, 19(5):445-456(2017) [pubmed]

10.6019/PXD006036;

curator keyword: Biological, Biomedical

submitter keyword: acetylation, SIRT2, differentiation,human pluripotent stem cells

Kwang-Soo Kim
contact affiliation	Molecular Neurobiology Laboratory, Department of Psychiatry and McLean Hospital, Harvard Medical School
contact email	kskim@mclean.harvard.edu
lab head
Byung-gyu kim
contact affiliation	Center for genomic integrity (CGI), Institute of basic science (IBS), UNIST, Ulsan, 44919, Republic of Korea
contact email	goldenlion@ibs.re.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006036

PRIDE project URI

• PRIDE
  1. PXD006036
     1. Label: PRIDE project
     2. Name: Metabolic control of primed human pluripotent stem cell fate and function by the miR-200c-SIRT2 axis