PXD006030-2

PXD006030 is an original dataset announced via ProteomeXchange.

Title	MULTI-TARGETED FLAVONOID INHIBITION OF THE PATHOGENIC BACTERIUM STAPHYLOCOCCUS AUREUS: A Proteomic Characterization
Description	Growth inhibition of the pathogen Staphylococcus aureus with currently available antibiotics is problematic in part due to bacterial biofilm protection. While Although the recently characterized natural products, including 3´,4´,5-trihydroxy-6,7-dimethoxy-flavone [1], 3´,4´,5,6,7-pentahydroxy-flavone [2] and 5-hydroxy-4´,7-dimethoxy-flavone [3] exhibit both antibiotic and biofilm inhibitory activities, the mode of action of such hydroxylated flavonoids with respect to S. aureus inhibition has is yet to be characterized. Enzymatic digestion and high- resolution MS mass spectral analysis of differentially expressed proteins from S. aureus, with and without exposure to antibiotic flavonoids (1-3) has allowed for the characterization of global -protein alterations induced by metabolite treatment. A total of 56, 92, and 110 proteins are were differentially expressed with bacterial exposure to 1, 2 or 3, respectively. Connectivity of identified proteins is characterized using a search tool for the retrieval of interacting genes/proteins (STRING) with multi-targeted S. aureus inhibition of energy metabolism and biosynthesis by the assayed flavonoids observed. Identifying the mode of action of natural products as antibacterial agents is expected to provide insight into the potential use of flavonoids alone or in combination with known therapeutic agents to effectively control S. aureus infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:16.417.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenjing Peng
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-06 03:45:32	ID requested
1	2017-05-30 00:19:28	announced
⏵ 2	2024-10-22 04:07:22	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00137;

Elmasri WA, Zhu R, Peng W, Al-Hariri M, Kobeissy F, Tran P, Hamood AN, Hegazy MF, Par, é PW, Mechref Y, Multitargeted Flavonoid Inhibition of the Pathogenic Bacterium Staphylococcus aureus: A Proteomic Characterization. J Proteome Res, 16(7):2579-2586(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: antibacterial flavonoids

LC-MS/MS proteomics

multi-targeted bacterial inhibition,Staphylococcus aureus

Yehia Mechref
contact affiliation	Department of Chemistry and Biochemistry, Texas Tech University
contact email	yehia.mechref@ttu.edu
lab head
Wenjing Peng
contact affiliation	Texas Tech University
contact email	wenjing.peng@ttu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD006030

PRIDE project URI

• PRIDE
  1. PXD006030
     1. Label: PRIDE project
     2. Name: MULTI-TARGETED FLAVONOID INHIBITION OF THE PATHOGENIC BACTERIUM STAPHYLOCOCCUS AUREUS: A Proteomic Characterization