PXD006028-1

PXD006028 is an original dataset announced via ProteomeXchange.

Title	Condensin chromatin association is regulated by the combined actions of SMC head and hinge engagement and phosphorylation
Description	The SMC condensin complex is essential for normal mitotic chromosome structure in eukaryotes. Here we undertake a comprehensive analysis of the importance of the condensin ATP binding and hydrolysis, SMC hinge stability and condensin subunit phosphorylation for chromatin association in budding yeast. We demonstrate that ATP binding is required for condensin chromatin association by both promoting stable binding of Smc4 and enabling head-head engagement. In contrast destabilizing the hinge domain of condensin facilitates chromatin association. Phosphorylation of condensin is enriched in enzymatic states with elevated chromatin association. Furthermore, condensin phosphorylation and Aurora/Ipl1 kinase function are required to maintain mitotic chromatin association. We find that Ipl1 activity stabilizes Smc4 interaction within the complex in a similar manner to ATP binding, illustrating a novel pathway of regulation of condensin activity by phosphorylation. In summary condensin’s chromatin association is positively regulated by phosphorylation and SMC ATP binding dependent head engagement and negatively regulated by hinge engagement. Our data argues that control of chromatin association by phosphorylation is through regulation of specific steps of its enzymatic cycle.
HostingRepository	PRIDE
AnnounceDate	2025-10-14
AnnouncementXML	Submission_2025-10-14_06:37:46.298.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steve Sweet
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-06 03:07:01	ID requested
⏵ 1	2025-10-14 06:37:46	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Cerevisiae yeast condensin phosphorylation

Jon Baxter
contact affiliation	Genome Damage and Stability Centre, Science Park Road, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, U.K.
contact email	Jon.Baxter@sussex.ac.uk
lab head
Steve Sweet
contact affiliation	NantOmics
contact email	stephen.sweet@nantomics.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006028
     1. Label: PRIDE project
     2. Name: Condensin chromatin association is regulated by the combined actions of SMC head and hinge engagement and phosphorylation