PXD006025-2

PXD006025 is an original dataset announced via ProteomeXchange.

Title	hickory LC-MS/MS - Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process
Description	Hickory (Carya cathayensis) is a woody plant with high nutritional and economic value, and is widely cultivated in China. It is different from herbaceous plants and undergoes a long vegetative stage before reaching floral transition. Hickory can be cultivated on a large scale using grafting. To reveal the response mechanisms to grafting, we employed a proteomics-based approach to identify differentially expressed proteins during the hickory grafting process. In our study, 3723 proteins were identified and 2518 proteins were quantified. A total of 710 differentially quantified proteins (DQPs) were identified and these were involved in various molecular functional and biological processes. Among these DQPs, 341 were up-regulated and 369 were down-regulated at 7 days after grafting compared to the control. The protein interaction networks of the DQPs indicated that there were two enriched interaction clusters. These were „ribosome‟ and „starch and sucrose metabolism‟. Four auxin-related proteins were down-regulated, which was in agreement with the transcription levels of their encoding genes. The Kyoto Encyclopedia of Genes and Genomes analysis showed that the „Flavonoid biosynthesis‟ pathway was significantly up-regulated. Further experiments verified a significant increase in the total flavonoid contents in scions, which suggested that graft union formation may activate flavonoid biosynthesis to increase the contents of a series of downstream secondary metabolites. This comprehensive analysis provides fundamental information on the candidate proteins and the secondary metabolism pathways involved in the grafting process for hickory.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:59.961.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhang zhang
SpeciesList	scientific name: Carya cathayensis; NCBI TaxID: 139927;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-06 01:31:36	ID requested
1	2018-10-26 09:19:32	announced
⏵ 2	2024-10-22 04:07:00	announced	2024-10-22: Updated project metadata.

Xu D, Yuan H, Tong Y, Zhao L, Qiu L, Guo W, Shen C, Liu H, Yan D, Zheng B, ) Provides Insights into Response Mechanisms to Grafting Process. Front Plant Sci, 8():676(2017) [pubmed]

10.3389/fpls.2017.00676;

curator keyword: Biological

submitter keyword: flavonoid biosynthesis

graft

hickory

TMT

secondary metabolism

Bingsong Zheng
contact affiliation	Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A 20 & F University, Dong Hu Campus, 88 Northern Circle Road, Linan 311300, China.
contact email	bszheng@zafu.edu.cn
lab head
zhang zhang
contact affiliation	Hangzhou
contact email	data5499tq@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006025

PRIDE project URI

• PRIDE
  1. PXD006025
     1. Label: PRIDE project
     2. Name: hickory LC-MS/MS - Comparative Proteomic Analysis of the Graft Unions in Hickory (Carya cathayensis) Provides Insights into Response Mechanisms to Grafting Process