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PXD005994-3

PXD005994 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAspergillus fumigatus melanin manipulates the cargo and kinetics of neutrophil-derived extracellular vesicles
DescriptionNeutrophil-derived extracellular vesicles have regained scientific interest as potent ‘wireless’ modulators with pleiotropic effects on the immune system. A few studies have addressed their role in the context of microbial pathogenesis showing their bacteriostatic effects against Staphylococcus aureus in vitro and their potential as diagnostic markers in the onset of bacterimic sepsis in vivo. Here, we provided first insights into the vesicle release driven by the clinically relevant pathogenic fungus Aspergillus fumigatus that causes invasive infections in immunocompromised individuals worldwide often with a lethal outcome. Neutrophils, stimulated with fungal spores, potently produced vesicles that we refer as anti-fungal extracellular vesicles (afEVs). We revealed that the conidial surface pigment of A. fumigatus dihydroxynaphthalene (DHN) melanin - a well known fungal virulence factor - is a key ‘switch-on/off’ governing vesicle production. We profiled the kinetics of afEV formation and showed that DHN-melanin interferes with the vesicle number, vesicle surface maturation markers such as CD11b, CD63 and CD177 and governs the enrichment of distinct afEV subpopulations unrelated to apoptosis. Simultaneously we tracked down the fate of neutrophils during afEV formation and observed mulivesicular body formation that led to active export of CD11b, CD63 and CD177 by shedding from the surface of neutrophils onto the surface afEVs. By dissecting the proteome cargo of afEVs produced against wild-type and melanin-free conidia we verified that DHN-melanin also manipulates the type of cargo neutrophils accumulate in the vesicles upon confronting conidia with different cell wall integrity. afEVs contained higher amounts of antimicrobial peptides than spontaneously released EVs from uninfected neutrophils. Last but not least afEVs exhibit fungistatic effects and also modulate cell wall morphogenesis of hyphae.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:36:23.478.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Krüger
SpeciesList scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-03-01 05:40:07ID requested
12019-05-17 09:30:43announced
22020-08-25 06:22:25announced2020-08-25: Updated publication reference for PubMed record(s): 32291301.
32024-10-22 04:36:28announced2024-10-22: Updated project metadata.
Publication List
Shopova IA, Belyaev I, Dasari P, Jahreis S, Stroe MC, Cseresny, é, s Z, Zimmermann AK, Medyukhina A, Svensson CM, Kr, ü, ger T, Szeifert V, Nietzsche S, Conrad T, Blango MG, Kniemeyer O, von Lilienfeld-Toal M, Zipfel PF, Ligeti E, Figge MT, Brakhage AA, Human Neutrophils Produce Antifungal Extracellular Vesicles against Aspergillus fumigatus. mBio, 11(2):(2020) [pubmed]
10.1128/mbio.00596-20;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: antifungal extracellular vesicles, Aspergillus fumigatus, melanin,neutrophils
Contact List
Axel A. Brakhage
contact affiliationInstitute for Microbiology, Friedrich Schiller University, Jena, Germany and Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product and Infection Biology (HKI), Hans Knöll Institute, Jena, Germany
contact emailaxel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliationLeibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact emailthomas.krueger@leibniz-hki.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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