PXD005968 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Prediction of protein complexes in Trypanosoma brucei by protein correlation profiling mass spectrometry and machine learning |
Description | A disproportionate number of predicted proteins from the genome sequence of the protozoan parasite Trypanosoma brucei, an important human and animal pathogen, are hypothetical proteins of unknown function. This work describes a protein correlation profiling mass spectrometry approach, using two size exclusion and one ion exchange chromatography systems, to derive sets of predicted protein complexes in this organism by hierarchical clustering and machine learning methods. We provide examples of both potential new subunits of known protein complexes and of novel trypanosome complexes of suggested function, contributing to improving the functional annotation of the trypanosome proteome. These hypothesis-generating proteomic data are provided in an open access online data visualisation environment (http://134.36.66.166:8083/complex_explorer) |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-20 |
AnnouncementXML | Submission_2017-10-20_02:12:25.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Crozier |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-02-27 01:27:45 | ID requested | |
⏵ 1 | 2017-10-20 02:12:27 | announced | |
Publication List
Crozier TWM, Tinti M, Larance M, Lamond AI, Ferguson MAJ, by Protein Correlation Profiling Mass Spectrometry and Machine Learning. Mol Cell Proteomics, 16(12):2254-2267(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Trypanosoma, procyclic, protein correlation profiling mass spectrometry, protein complexes |
Contact List
Angus Lamond |
contact affiliation | Univeristy of Dundee |
contact email | a.i.lamond@dundee.ac.uk |
lab head | |
Thomas Crozier |
contact affiliation | University of Cambridge |
contact email | t.monteirocrozier@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD005968 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005968
- Label: PRIDE project
- Name: Prediction of protein complexes in Trypanosoma brucei by protein correlation profiling mass spectrometry and machine learning