PXD005943 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Saccharomyces cerevisiae mRBPome in oxidative stress conditions |
Description | This project aims to identify novel RNA binding proteins in the baker's yeast , Saccharomyces cerevisiae, involved in the oxidative stress,. Since interactions between RNAs and proteins may be transient, yeast cells, either untreated and growth in rich media and exposed to 0.5 mM hydrogen peroxide for 15 minutes were crosslinked with UV light at 254 nm which promotes the covalent link between proteins and RNAs. After this, polyadenylated mRNAs were purified via oligo(dT) coupled to magentic beads under stringent conditions. Finally, samples were subjected to mass spectrometry analysis. To rule out the possibility of RNA-independent binding we also analysed other samples where we performed competition assays with polyadenylic acid |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-10 |
AnnouncementXML | Submission_2022-03-14_04:26:22.644.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ana Matia Gonzalez |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-02-21 05:04:14 | ID requested | |
1 | 2021-06-09 22:01:29 | announced | |
⏵ 2 | 2022-03-14 04:26:23 | announced | 2022-03-14: Updated publication reference for PubMed record(s): 34278261. |
Publication List
Matia-Gonz, รก, lez AM, Jabre I, Laing EE, Gerber AP, Oxidative stress induces coordinated remodeling of RNA-enzyme interactions. iScience, 24(7):102753(2021) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Saccharomyces cerevisiae, RBP, LC-MS/MS, hydrogen peroxide, oxidative stress |
Contact List
Andre Paul Gerber |
contact affiliation | Dept. of Microbial Sciences School of Biosciences and Medicine Faculty of Health and Medical Sciences University of Surrey Stag Hill Campus 19 AX 01 Guildford, GU2 7XH U.K. |
contact email | a.gerber@surrey.ac.uk |
lab head | |
Ana Matia Gonzalez |
contact affiliation | University of Surrey |
contact email | a.matia@surrey.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005943
- Label: PRIDE project
- Name: Saccharomyces cerevisiae mRBPome in oxidative stress conditions