PXD005913 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nuclear poly(A)-binding protein 1 is an ATM target and essential for DNA double-strand break rep |
Description | The DNA damage response (DDR) is an extensive signaling network that is robustly mobilized by DNA double-strand breaks (DSBs). The primary transducer of the DSB response is the protein kinase, ataxia-telangiectasia, mutated (ATM). Here, we establish nuclear poly(A)-binding protein 1 (PABPN1) as a novel target of ATM and a crucial player in the DSB response. PABPN1 usually functions in regulation of RNA processing and stability. We establish that PABPN1 is recruited to the DDR as a critical regulator of DSB repair. A portion of PABPN1 relocalizes to DSB sites and is phosphorylated on Ser95 in an ATM-dependent manner. PABPN1 depletion sensitizes cells to DSBinducing agents and prolongs the DSB-induced G2/M cell-cycle arrest, and DSB repair is hampered by PABPN1 depletion or elimination of its phosphorylation site. PABPN1 is required for optimal DSB repair via both nonhomologous end-joining (NHEJ) and homologous recombination repair (HRR), and specifically is essential for efficient DNAend resection, an initial, key step in HRR. Using mass spectrometry analysis, we capture DNA damage-induced interactions of phospho-PABPN1, including well-established DDR players as well as other RNA metabolizing proteins. Our results uncover a novel ATM-dependent axis in the rapidly growing interface between RNA metabolism and the DDR. |
HostingRepository | PRIDE |
AnnounceDate | 2017-11-30 |
AnnouncementXML | Submission_2017-11-30_01:41:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tamar Geiger |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-02-13 01:14:54 | ID requested | |
⏵ 1 | 2017-11-30 01:41:11 | announced | |
2 | 2018-05-23 06:58:41 | announced | Updated publication reference for PubMed record(s): 29253183. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: PABPN1, mass spectrometry, LC-MS/MS, DNA damage response |
Contact List
Tamar Geiger |
contact affiliation | Department of Human Molecular Genetics and Biochemistry Sackler Faculty of Medicine Tel Aviv University Israel |
contact email | geiger@tauex.tau.ac.il |
lab head | |
Tamar Geiger |
contact affiliation | Tel Aviv University |
contact email | geiger@tauex.tau.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005913
- Label: PRIDE project
- Name: Nuclear poly(A)-binding protein 1 is an ATM target and essential for DNA double-strand break rep