PXD005904-1

PXD005904 is an original dataset announced via ProteomeXchange.

Title	UBE2O remodels the proteome during terminal erythroid differentiation
Description	Terminal differentiation is characterized by a respecification of the global protein complement, as epitomized by erythrocytes, whose cytosol is ~98% globin. Remodeling of the proteome involves programmed elimination of generic cellular constituents in parallel with synthesis of cell-type-specific proteins. The erythroid proteome undergoes a rapid transition at the reticulocyte stage; however, the mechanisms driving elimination of preexisting cytosolic proteins are unidentified. UBE2O is a ubiquitin-conjugating enzyme expressed at elevated levels during late stage erythropoiesis. A Ube2o null mutation in mice results in anemia. Proteomic analysis of this mutant suggests that UBE2O is a broad-spectrum ubiquitinating enzyme that remodels the erythroid proteome. In particular, a hallmark of the reticulocyte to mature erythrocyte transition—ribosome elimination—is defective in Ube2o-/- mutants. UBE2O recognizes ribosomal proteins and other substrates directly, targeting them to proteasomes for degradation. Thus, in reticulocytes, and perhaps other highly differentiated cells, the induction of ubiquitinating factors may drive the transition from a complex to a simple proteome.
HostingRepository	PRIDE
AnnounceDate	2017-08-08
AnnouncementXML	Submission_2017-08-08_00:31:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Prado
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-10 03:06:32	ID requested
⏵ 1	2017-08-08 00:31:26	announced
2	2024-10-22 04:35:26	announced	2024-10-22: Updated project metadata.

Nguyen AT, Prado MA, Schmidt PJ, Sendamarai AK, Wilson-Grady JT, Min M, Campagna DR, Tian G, Shi Y, Dederer V, Kawan M, Kuehnle N, Paulo JA, Yao Y, Weiss MJ, Justice MJ, Gygi SP, Fleming MD, Finley D, UBE2O remodels the proteome during terminal erythroid differentiation. Science, 357(6350):(2017) [pubmed]

curator keyword: Biological

submitter keyword: UBE2O, red blood cell, proteomics, ubiquitin, proteasome, TMT, MS3

Daniel J Finley
contact affiliation	Finley and Gygi lab Cell Biology Department Harvard Medical School US
contact email	daniel_finley@hms.harvard.edu
lab head
Miguel Prado
contact affiliation	Harvard Medical School
contact email	miguel_prado@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD005904

PRIDE project URI

• PRIDE
  1. PXD005904
     1. Label: PRIDE project
     2. Name: UBE2O remodels the proteome during terminal erythroid differentiation