PXD005890 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improving Mass Spectrometer Acquisition when using Tandem MS/MS/MS Scanning with Isobaric Tags |
Description | n this work we utilize a standard, high-complexity Human cell line tryptic digest combined with a synthetic peptide library to systematically evaluate the properties of a variety of MS operation modes and detector configurations for the optimal examination of isobaric-tagged peptide mixtures. We demonstrate that despite the ability of the IT to yield more rapid MS3 scanning, the benefit in peptide and protein identifications when examining complex mixtures is minor while the negative impact on quantification precision is significant. |
HostingRepository | PRIDE |
AnnounceDate | 2017-04-26 |
AnnouncementXML | Submission_2017-04-26_04:50:54.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christopher Hughes |
SpeciesList | scientific name: Ralstonia eutropha JMP134; NCBI TaxID: 264198; scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-02-09 01:36:43 | ID requested | |
⏵ 1 | 2017-04-26 04:50:55 | announced | |
Publication List
Hughes CS, Spicer V, Krokhin OV, Morin GB, Investigating Acquisition Performance on the Orbitrap Fusion When Using Tandem MS/MS/MS Scanning with Isobaric Tags. J Proteome Res, 16(5):1839-1846(2017) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: TMT, MS3, Orbitrap Fusion |
Contact List
Gregg Morin |
contact affiliation | Head of Proteomics |
contact email | gmorin@bcgsc.ca |
lab head | |
Christopher Hughes |
contact affiliation | British Columbia Cancer Research Centre |
contact email | chughes@bcgsc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005890 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005890
- Label: PRIDE project
- Name: Improving Mass Spectrometer Acquisition when using Tandem MS/MS/MS Scanning with Isobaric Tags