PXD005859-1

PXD005859 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomics of Flagellin C9LY14 from Selenomonas sputigena
Description	The Gram-negative, flagellated, anaerobic, crescent-shaped bacterium Selenomonas sputigena is a potential human periodontal pathogen. Information on its virulence factors and underlying pathogenicity mechanisms is scarce. Here we show for the first time that S. sputigena produces a diversely and heavily O-glycosylated flagellin C9LY14 as a major cellular protein, which carries various hitherto undescribed rhamnose- and N acetylglucosamine-linked O-glycans in the range from mono- to hexasaccharides. A comprehensive glycomic and glycoproteomic assessment revealed extensive glycan macro- and microheterogeneity on 20 unique glycopeptide species. From the multiple sites of glycosylation, five were unambiguously identified on the 437-amino acid C9LY14 protein (Thr149, Ser182, Thr199, Thr259, and Ser334). The O-glycans additionally showed modifications by methylation and acetylation. This is the first report on O-linked flagellin glycosylation in S. sputigena, revealing that C9LY14 is one of the most heavily glycosylated flagellins described to date.
HostingRepository	PRIDE
AnnounceDate	2018-01-24
AnnouncementXML	Submission_2018-01-25_01:30:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Kolarich
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Selenomonas sputigena ATCC 35185; NCBI TaxID: 546271;
ModificationList	N-acetylhexosaminylated; monohydroxylated residue
Instrument	amaZon Speed ETD

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-06 03:34:32	ID requested
⏵ 1	2018-01-25 01:30:06	announced
2	2024-10-22 04:35:32	announced	2024-10-22: Updated project metadata.

Rath CB, Schirmeister F, Figl R, Seeberger PH, Sch, ä, ffer C, Kolarich D, -glycans and Rhamnose Fragment Rearrangement Occurring on the Glycopeptides. Mol Cell Proteomics, 17(4):721-736(2018) [pubmed]

curator keyword: Biological

submitter keyword: Selenomonas sputigena, flagellin glycosylation, periodontitis, glycoproteomics, glycomics, LC-MSMS

Associate Professor Daniel Kolarich, PhD
contact affiliation	Institute for Glycomics, Gold Coast Campus, Griffith University, QLD 4222, Australia
contact email	d.kolarich@griffith.edu.au
lab head
Daniel Kolarich
contact affiliation	Griffith University, Institute for Glycomics
contact email	d.kolarich@griffith.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005859
     1. Label: PRIDE project
     2. Name: Glycoproteomics of Flagellin C9LY14 from Selenomonas sputigena