PXD005829-2

PXD005829 is an original dataset announced via ProteomeXchange.

Title	The 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels
Description	Deregulated expression of MYC enhances glutamine utilization and renders cell survival dependent on glutamine, inducing “glutamine addiction”. Surprisingly, colon cancer cells that express high levels of MYC due to WNT pathway mutations, are not glutamine-addicted but undergo a reversible cell cycle arrest upon glutamine deprivation. We show here that glutamine deprivation suppresses translation of endogenous MYC via the 3’-UTR of the MYC mRNA, enabling escape from apoptosis. This regulation is mediated by glutamine-dependent changes in adenosine nucleotide levels. Glutamine deprivation causes a global reduction in promoter association of RNA Polymerase II (RNAPII) and slows transcriptional elongation. While activation of MYC restores binding of MYC and RNAPII function on most promoters, restoration of elongation is imperfect and activation of MYC in the absence of glutamine causes stalling of RNAPII on multiple genes, correlating with R-loop formation. Stalling of RNAPII and R-loop formation can cause DNA damage, arguing that the MYC 3’-UTR is critical for maintaining genome stability when ribonucleotide levels are low.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:22.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Vanselow
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-01 06:43:54	ID requested
1	2022-02-28 03:02:59	announced
⏵ 2	2023-11-14 08:49:23	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: myc,mRNA-protein interaction, glutamine, nanoLC-MS/MS

Andreas Schlosser
contact affiliation	Prof. Dr. Andreas Schlosser Rudolf-Virchow-Zentrum für Experimentelle Biomedizin Universität Würzburg Josef-Schneider-Str. 2 97080 Würzburg Germany
contact email	andreas.schlosser@uni-wuerzburg.de
lab head
Jens Vanselow
contact affiliation	University Wuerzburg
contact email	jens.vanselow@uni-wuerzburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD005829

PRIDE project URI

• PRIDE
  1. PXD005829
     1. Label: PRIDE project
     2. Name: The 3’-UTR of MYC couples RNA polymerase II function to ribonucleotide levels