PXD005825-2

PXD005825 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic approach identifies Vpr binding protein as novel host factor supporting influenza A virus infections in human cells
Description	Influenza A virus infections are a major cause for respiratory disease in humans, which affects all age groups and contributes substantially to global morbidity and mortality. IAV have a large natural host reservoir in avian species. However, many avian IAV strains lack adaptation to other hosts and hardly propagate in humans. While seasonal or pandemic influenza A virus (IAV) strains replicate efficiently in permissive human cells, many avian IAV cause abortive non-productive infections in these hosts despite successful cell entry. However, the precise reasons for these differential outcomes are poorly defined. We hypothesized that the distinct course of an IAV infection with a given virus strain is determined by the differential interplay between specific host and viral factors. By using Spike-in SILAC mass spectrometry-based quantitative proteomics we characterized sets of cellular factors whose abundance is specifically up- or down-regulated in the course of permissive vs. non-permissive IAV infection, respectively. This approach allowed for the definition and quantitative comparison of about 3500 proteins in human lung epithelial cells in response to seasonal or low-pathogenic avian H3N2 IAV. Many identified proteins were similarly regulated by both virus strains, but also 16 candidates with distinct changes in permissive vs. non-permissive infection were found. RNAi-mediated knockdown of these differentially regulated host factors identified Vpr binding protein (VprBP) as pro-viral host factor since its down-regulation inhibited efficient propagation of seasonal IAV while over-expression increased viral replication of both seasonal and avian IAV. These results not only show that there are similar differences in the overall changes during permissive and non-permissive imfluenza virus infections, but also provide a basis to evaluate VprBP as novel anti-IAV drug target.
HostingRepository	PRIDE
AnnounceDate	2017-03-14
AnnouncementXML	Submission_2017-03-16_05:10:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Saenger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine; monohydroxylated residue; acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-02-01 02:34:47	ID requested
1	2017-03-14 04:30:58	announced
⏵ 2	2017-03-16 05:10:20	announced	Updated publication reference for PubMed record(s): 28289176.
3	2017-06-21 00:31:35	announced	Updated project metadata.
4	2017-10-24 04:05:22	announced	Updated project metadata.
5	2024-10-22 04:36:02	announced	2024-10-22: Updated project metadata.

Sadewasser A, Paki K, Eichelbaum K, Bogdanow B, Saenger S, Budt M, Lesch M, Hinz KP, Herrmann A, Meyer TF, Karlas A, Selbach M, Wolff T, Quantitative Proteomic Approach Identifies Vpr Binding Protein as Novel Host Factor Supporting Influenza A Virus Infections in Human Cells. Mol Cell Proteomics, 16(5):728-742(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Influenza, quantitative proteomics, Vprbp

Thorsten Wolff
contact affiliation	Robert Koch Institute FG17 - Influenza and Other Respiratory Viruses Seestraße 10, 13353 Berlin, Germany
contact email	wolfft@rki.de
lab head
Sandra Saenger
contact affiliation	Robert Koch Institute
contact email	saengers@rki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005825

PRIDE project URI

• PRIDE
  1. PXD005825
     1. Label: PRIDE project
     2. Name: Quantitative proteomic approach identifies Vpr binding protein as novel host factor supporting influenza A virus infections in human cells