PXD005772-1

PXD005772 is an original dataset announced via ProteomeXchange.

Title	MALDI Quant Fibrinogen
Description	Absolute quantification of protein was performed by MALDI-LTQ using scramble internal standard (sIS) peptide. Mixtures of serial dilutions of fibrinogen peptides (final concentration: 50, 100, 250, 500 and 1000 fmol/μL), equal amounts of corresponding sIS peptides (final concentration: 500 fmol/μL) and serial dilutions of digested serum (final concentration: 0, 0.025, 0.05, 0.25 and 0.5 μg/μL) were analyzed by MALDI-MS/MS. We also analyzed immunoprecipitated (IP) α-fibrinogen in 0.05-5 μL of plasma by MALDI-MS/MS.
HostingRepository	jPOST
AnnounceDate	2017-04-08
AnnouncementXML	Submission_2017-04-07_08:00:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sumio Ohtsuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; (2S,3R)-3-hydroxyaspartic acid; L-aspartic 4-phosphoric anhydride
Instrument	MALDI LTQ XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-24 00:31:54	ID requested
⏵ 1	2017-04-07 08:00:06	announced
2	2018-09-18 23:58:24	announced	Updated CV, FTP location and Modification list.
3	2021-02-04 17:17:10	announced	2021-02-04: Updated FTP location.
4	2022-09-18 04:06:52	announced	2022-09-18: Updated FTP location.

Yoneyama T, Ohtsuki S, Tachikawa M, Uchida Y, Terasaki T, Scrambled Internal Standard Method for High-Throughput Protein Quantification by Matrix-Assisted Laser Desorption Ionization Tandem Mass Spectrometry. J Proteome Res, 16(4):1556-1565(2017) [pubmed]

submitter keyword: MALDI Quant

Sumio Ohtsuki
lab head
Sumio Ohtsuki
contact affiliation	Kumamoto University
dataset submitter

jPOST dataset URI