PXD005753 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PTMs of HTT protein |
Description | Post-translational modifications (PTMs) of proteins regulate various cellular processes. PTMs of polyglutamine-expanded huntingtin (Htt) protein, causative of Huntington’s disease (HD), are likely modulators of HD pathogenesis. Previous studies have identified and characterized several PTMs on exogenously expressed Htt fragments, however none of these studies were designed to systematically characterize PTMs on the endogenous full-length Htt protein.We found that full-length endogenous Htt, immunoprecipitated from HD knock-in mouse and human post mortem brain, is suitable for detection of PTMs by mass spectrometry. Using label-free mass spectrometry, we identified around 40 PTMs, of which half are novel. These findings will be instrumental in the further assembling the Htt PTM framework, and validate PTMs of Htt as promising therapeutic targets for HD. |
HostingRepository | PRIDE |
AnnounceDate | 2017-06-29 |
AnnouncementXML | Submission_2017-06-29_06:14:47.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005753 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tamara Ratovitski |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | HNE; Hex; CAMthiopropanoyl; CarbamidomethylDTT; GlycerylPE; Propionamide; BADGE; Deamidated; sulfo+amino; Trp->Oxolactone; CHDH; Glucuronyl; Cation:K; NO_SMX_SMCT; Gln->pyro-Glu; Met->Hsl; Diethylphosphate; DAET; DeStreak; BHTOH; NA-LNO2; Trimethyl; Ammonia-loss; Ethylphosphate; Oxidation; AEC-MAEC; Carboxy; BITC; O-pinacolylmethylphosphonate; 4-ONE; PET; Ethanolamine; Cation:Na; PyMIC; Trp->Hydroxykynurenin; GlyGly; Deoxy; Pyro-carbamidomethyl; Formyl; Carbamyl; LG-anhydrolactam; Cation:Mg[II]; Thiophospho; Tyr->Dha; Ammonium; 2-succinyl; Cation:Cu[I]; Crotonaldehyde; Cytopiloyne; Dethiomethyl; Amidated; Thioacyl; Cys->Dha; Pro->pyro-Glu; Phospho; Hep; Cation:Zn[II]; PEITC; Acetyl; Galactosyl; Nitro; Methylthio; Carboxymethyl; HexNAc; Carbamidomethyl; 3sulfo; Cation:Ca[II]; Trioxidation; trifluoro; Glu->pyro-Glu; CarboxymethylDTT; MercaptoEthanol; Hex(1)HexNAc(1)NeuAc(1); EDT-maleimide-PEO-biotin; dHex; Delta:H(2)C(2); Ethyl; Cation:Fe[II]; O-Isopropylmethylphosphonate; Cyano; Methyl; AEBS; Pyridylacetyl; Methylmalonylation; Glucosylgalactosyl; Pyridylethyl; HNE-Delta:H(2)O; Didehydro; Cytopiloyne+water; Trp->Kynurenin; SMA; Iminobiotin; Iodo; Cys->Oxoalanine; SecCarbamidomethyl; Hydroxycinnamyl; Lys->Allysine; LG-anhyropyrrole; Amidine; Hex(1)HexNAc(1)NeuAc(2); Dehydrated; Biotin; Dioxidation; Benzoyl; glycidamide |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-01-20 04:32:18 | ID requested | |
⏵ 1 | 2017-06-29 06:14:48 | announced | |
2 | 2017-06-29 06:19:47 | announced | Updated publication reference for PubMed record(s): 2865385, 28653853. |
Publication List
Ando S, [Biochemistry of brain aging]. Nihon Rinsho, 43(7):1399-403(1985) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Huntingtin, HD, human and mouse brain |
Contact List
Christopher A. Ross |
contact affiliation | Division of Neurobiology, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, US |
contact email | caross@jhu.edu |
lab head | |
Tamara Ratovitski |
contact affiliation | JHU |
contact email | tratovi1@jhmi.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005753
- Label: PRIDE project
- Name: PTMs of HTT protein