PXD005718-1

PXD005718 is an original dataset announced via ProteomeXchange.

Title	Regulation of somatosensory mechanotransduction by Annexin A6
Description	Sensory neuron mechanically-activated slowly adapting currents have been linked to noxious mechanosensation. We identified a Conotoxin, Noxious Mechanosensation Blocker -1, that blocks such currents selectively. Using an active biotinylated form of the toxin we identified 67 binding proteins in sensory neurons and sensory neuron-derived cell lines using mass spectrometry. Annexin A6 was the most frequently identified binding protein. Annexin A6 knockout mice showed an enhanced sensitivity to mechanical stimuli. Rapidly adapting currents were enhanced and slowly adapting channels diminished. The percentage of neurons expressing slowly adapting currents fell and non-responsive neurons increased. Conversely, overexpression of annexin A6 in DRG neurons inhibited rapidly adapting currents without effects on slowly adapting currents. Co-expression of annexin A6 with Piezo 2 led to an inhibition of Piezo-mediated rapidly adapting currents. AAV-mediated gene delivery of annexin A6 to sensory neurons in a mouse model of osteoarthritis attenuated mechanical pain. These data demonstrate a role for annexin A6 in somatosensory mechanotransduction.
HostingRepository	PRIDE
AnnounceDate	2018-04-17
AnnouncementXML	Submission_2018-04-17_01:06:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005718
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	James Wright
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Deamidated; Oxidation; Acetyl; Oxidation
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-16 11:19:20	ID requested
⏵ 1	2018-04-17 01:06:33	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Mouse, Brain, LTQ-FT, ND-C Cells

Jyoti Choudhary
contact affiliation	Wellcome Trust Sanger Institute
contact email	jc4@sanger.ac.uk
lab head
James Wright
contact affiliation	Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute
contact email	jw13@sanger.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005718
     1. Label: PRIDE project
     2. Name: Regulation of somatosensory mechanotransduction by Annexin A6