PXD005699 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger |
Description | Renewables-based biotechnology depends on enzymes to degrade plant lignocellulose to simple sugars that are converted to fuels or high-value products. Identification and characterization of such lignocellulose degradative enzymes could be fast-tracked by availability of an enzyme activity measurement method that is fast, label-free, uses minimal resources and allows direct identification of generated products. We developed such a method by applying carbohydrate arrays coupled with MALDI-ToF mass spectrometry to identify reaction products of carbohydrate active enzymes (CAZymes) of the filamentous fungus Aspergillus niger. We describe the production and characterization of plant polysaccharide-derived oligosaccharides and their attachment to hydrophobic self-assembling monolayers on a gold target. We verify effectiveness of this array for detecting exo- and endo-acting glycoside hydrolase activity using commercial enzymes, and demonstrate how this platform is suitable for detection of enzyme activity in relevant biological samples, the culture filtrate of A. niger grown on wheat straw. In conclusion, this versatile method is broadly applicable in screening and characterisation of activity of CAZymes, such as fungal enzymes for plant lignocellulose degradation with relevance to biotechnological applications as biofuel production, the food and animal feed industry. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-26 |
AnnouncementXML | Submission_2018-10-26_10:35:56.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005699 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jolanda van Munster |
SpeciesList | scientific name: Aspergillus niger; NCBI TaxID: 5061; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-01-12 03:20:20 | ID requested | |
⏵ 1 | 2018-10-26 10:35:57 | announced | |
Publication List
van Munster JM, Thomas B, Riese M, Davis AL, Gray CJ, Archer DB, Flitsch SL, Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger. Sci Rep, 7():43117(2017) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: Aspergillus niger, plant-polysaccharide degrading enzymes, CAZymes, lignocellulose |
Contact List
David Archer |
contact affiliation | University of Nottingham, School of Life Sciences |
contact email | David.Archer@Nottingham.ac.uk |
lab head | |
Jolanda van Munster |
contact affiliation | University of Manchester |
contact email | Jolandavanmunster@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD005699
- Label: PRIDE project
- Name: Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger