PXD005688-1

PXD005688 is an original dataset announced via ProteomeXchange.

Title	Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections
Description	Neutrophils have an important role in rapid antimicrobial defenses against and resolution of urinary tract infections (UTIs). We show that a mechanism known as neutrophil extracellular trap (NET) formation is a strategy to combat pathogens in the urinary tract. Viscous aggregates that were present in human urine sediments revealed high extracellular DNA content. The DNA formed a scaffold to which antimicrobial effector proteins derived from different neutrophil subcellular compartments bound. Treatment with deoxyribonuclease I solubilized these structures. We observed massive proteolytic degradation in the NETs with apparently dominant roles for the neutrophil granule proteases elastase, proteinase 3, and cathepsin G. In a UTI case with Staphylococcus aureus as the infectious agent, high abundance of autolysins and immune evasion factors in a cell-free NET extract suggested that controlled cell wall autolysis plays a role in derailing the host immune response and allows some bacterial cells to survive following entrapment in NETs.
HostingRepository	PRIDE
AnnounceDate	2017-01-23
AnnouncementXML	Submission_2017-01-24_01:18:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanbao Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-11 02:36:36	ID requested
⏵ 1	2017-01-24 01:18:49	announced
2	2017-02-02 05:56:49	announced	Updated publication reference for PubMed record(s): 28129394.
3	2024-10-22 04:34:59	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Urine, Proteomics, LCMSMS, Neutrophil Extracellular Traps (NETs), Urinary Tract Infection (UTI)

Yanbao Yu
contact affiliation	J. Craig Venter Institute, Rockville MD 20850 USA
contact email	yayu@jcvi.org
lab head
Yanbao Yu
contact affiliation	J. Craig Venter Institute
contact email	yayu@jcvi.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005688
     1. Label: PRIDE project
     2. Name: Characterization of Early-Phase Neutrophil Extracellular Traps in Urinary Tract Infections