PXD005636-1

PXD005636 is an original dataset announced via ProteomeXchange.

Title	Analysis of human AKT PH domain using limited proteolysis (LiP) followed by LC-MS/MS
Description	We identified that a lncRNA named Long Intergenic Noncoding RNA for Kinase Activation (LINK-A, also LINC01139) directly and specifically interacts with AKT and PIP3. The LINK-A-AKT and -PIP3 interactions facilitate AKT recruitment and consequent activation. we also identified the single nucleotides of LINK-A required for PIP3 and AKT binding respectively. We hypothesized that the formation of LINK-A-PIP3-AKT complex may cause potential conformational changes of AKT PH domain, which can be studied by limited proteolysis. We applied limited proteolysis (LiP) followed by liquid chromatography-mass spectrometry (LC-MS/MS) analysis to analyze the AKT PH domain in the absence or presence of PIP3 and/or LINK-A RNA.
HostingRepository	PRIDE
AnnounceDate	2017-02-10
AnnouncementXML	Submission_2017-02-10_08:44:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chunru lin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-01-03 01:25:29	ID requested
⏵ 1	2017-02-10 08:44:48	announced
2	2024-10-22 04:32:31	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Human, AKT, PH domain, PIP3, LiP, LC-MS/MS

Liuqing Yang
contact affiliation	Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center
contact email	lyang7@mdanderson.org
lab head
chunru lin
contact affiliation	MDACC
contact email	clin2@mdanderson.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005636

PRIDE project URI

• PRIDE
  1. PXD005636
     1. Label: PRIDE project
     2. Name: Analysis of human AKT PH domain using limited proteolysis (LiP) followed by LC-MS/MS