PXD005635 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Detecting Proteins Glycosylation by a Homogeneous Reac-tion System with Zwitterionic Gold Nanoclusters |
Description | Homogeneous gold nanoclusters (Au NCs) have been widely utilized in drug delivery, chemical sensing, bioassays and bio-labeling due to their unique physical and chemical properties. However, few attentions have been paid on their application in detecting protein post-translational modifications. Herein, we developed a homogeneous reaction system with water-soluble zwitterionic Au NCs to capture glycopeptides from the complex biological samples for the first time. The unique characteristics of Au NCs, such as the molecular-like property, the excellent homogeneity in aqueous solution and organic solvent responsive precipitation, the easy preparation in only 4.5 hours, contribute to the high efficiency and high through-put for capturing the targeted glycopeptides. Briefly, the Au NCs and tryptic protein samples were incubated together in a homogeneous aqueous solution and then the glycopeptides were collected by co-precipitation with zwitterionic Au NCs dur-ing gradually dropping addition of acetonitrile. Compared with the conventional heterogeneous system with solid-state ad-sorbents, the number of characterized glycosylation sites was improved 35%. Finally, the MS detection limitation as low as 50 amol was achieved for the tryptic digests of standard glycoprotein (IgG) and 1576 glycosylation sites from 713 glycopro-teins were feasibly identified from only 60 μg mouse liver proteins |
HostingRepository | PRIDE |
AnnounceDate | 2017-03-29 |
AnnouncementXML | Submission_2017-03-29_00:53:23.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jinan Li |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-01-03 01:12:19 | ID requested | |
⏵ 1 | 2017-03-29 00:53:24 | announced | |
Publication List
Li J, Liu J, Liu Z, Tan Y, Liu X, Wang F, Detecting Proteins Glycosylation by a Homogeneous Reaction System with Zwitterionic Gold Nanoclusters. Anal Chem, 89(8):4339-4343(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: gold cluster, glycopeptides, homogeneous reaction system |
Contact List
Fangjun Wang |
contact affiliation | Dalian Institute of Chemical Physics |
contact email | wangfj@dicp.ac.cn |
lab head | |
Jinan Li |
contact affiliation | Dalian Institute of Chemical Physics |
contact email | lijinan0824@dicp.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005635
- Label: PRIDE project
- Name: Detecting Proteins Glycosylation by a Homogeneous Reac-tion System with Zwitterionic Gold Nanoclusters