PXD005527 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Unbiased proteomic study of the axons of cultured rat cortical neurons |
Description | We have conducted quantitative proteomic analyses of the axons of cultured rat cortical neurons. Axons are isolated by using glass chips that enable the axons and their cell bodies of neurons to grow in separated regions on the chip surface. Proteins extracted from the isolated axons, as well as those extracted from whole cortical neurons are subjected to two-dimensional liquid chromatography (2D-LC)-mass spectrometry (MS)-MS analyses. The abundances of proteins in the axon are found to be strongly correlated with their average abundances in whole neurons. Based upon these data, a quantitative description of the protein distribution among various subcellular structures in the axon has been generated. The proteins extracted from the axons and whole neurons are also subjected to stable isotope dimethyl labeling reaction and then to 2D-LC-MS/MS analysis. Proteins enriched in the axon compartment of rat cortical neurons are thus identified. |
HostingRepository | PRIDE |
AnnounceDate | 2018-04-17 |
AnnouncementXML | Submission_2018-04-17_00:27:20.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chih-Fan Chuang |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-12-08 01:58:45 | ID requested | |
⏵ 1 | 2018-04-17 00:27:21 | announced | |
Publication List
Chuang CF, King CE, Ho BW, Chien KY, Chang YC, Unbiased Proteomic Study of the Axons of Cultured Rat Cortical Neurons. J Proteome Res, 17(5):1953-1966(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Rat, 2D-LC-MSMS, Axon, Label-free quantification, Dimethyl-labeling quantitative proteomics |
Contact List
Yen-Chung Chang |
contact affiliation | Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu, Taiwan, Republic of China |
contact email | ycchang@life.nthu.edu.tw |
lab head | |
Chih-Fan Chuang |
contact affiliation | Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, Republic of China |
contact email | ion516@yahoo.com.tw |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005527
- Label: PRIDE project
- Name: Unbiased proteomic study of the axons of cultured rat cortical neurons