PXD005523-1

PXD005523 is an original dataset announced via ProteomeXchange.

Title	A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
Description	Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom of 200 spiders was extracted and fractioned into aliquots greater than or less than 10kDa. Then, each aliquot was further aliquoted and digested with trypsin (4h), trypsin (18h), Pepsin (18h), Chymotrypsin (18h), and analyzed by MudPIT on an Orbitrap XL mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain de meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of this venom.
HostingRepository	PRIDE
AnnounceDate	2017-05-16
AnnouncementXML	Submission_2017-05-16_08:12:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulo Carvalho
SpeciesList	scientific name: Loxosceles intermedia; NCBI TaxID: 58218;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-07 07:38:08	ID requested
⏵ 1	2017-05-16 08:12:35	announced
2	2019-02-12 08:13:08	announced	Updated publication reference for PubMed record(s): 28696408.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: venom loxosceles intermedia

Paulo Costa Carvalho
contact affiliation	Computational Mass Spectrometry & Proteomics Group Carlos Chagas Institute, Fiocruz - PR Brazil
contact email	paulo@pcarvalho.com
lab head
Paulo Carvalho
contact affiliation	Laboratory for proteomics and protein engineering
contact email	paulo@pcarvalho.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005523

PRIDE project URI

• PRIDE
  1. PXD005523
     1. Label: PRIDE project
     2. Name: A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom