PXD005487-2

PXD005487 is an original dataset announced via ProteomeXchange.

Title	Proteome of Hypoxic Glioblastoma Cells
Description	Tumors are known for hypoxic microenvironment due to decreased hemoglobin oxygen saturation arising from high acidity in tumor tissue, high consumption rates of rapidly proliferating cells, and irregular or even short-time cessation of blood flow in deformed vessels. Consequently, hypoxia promotes tumor to be highly malignant, metastatic, and resistant to therapy. In order to better understand the mechanisms of the correlation between hypoxia and cancer progression, we have studied the proteome of glioblastoma cells grown for five successive days under 1% oxygen via an innovative strategy, which involves quantification of newly synthesized proteins from cells previously labeled with heavy stable-isotope arginine and real-time accurate assessment of cell replication through the arginine light/heavy ratio in histone peptides. We found that hypoxia affects cancer cells in three sequential stages in vitro: the early inflammatory response stage with highly over-expressed glucose transporter protein GLUT1 and inflammation marker MKP2; the pre-EMT stage with over-expression of literally all enzymes in the glycolysis pathway towards pyruvate, as well as vitamin B12 transporter protein TCN2 which is essential for one-carbon metabolism; and the last EMT stage with distinct morphologic changes and protein expression profiles. Expression of methylation enzymes and the cellular production of their cofactors are either revealed or predicted to be significantly influenced by hypoxia, which demonstrates the importance of a follow-up study on epigenetic reprogramming in hypoxic cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:58.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kangling Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-02 01:58:27	ID requested
1	2017-09-12 01:19:18	announced
⏵ 2	2024-10-22 04:34:01	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra117.000154;

Zhang K, Xu P, Sowers JL, Machuca DF, Mirfattah B, Herring J, Tang H, Chen Y, Tian B, Brasier AR, Sowers LC, Proteome Analysis of Hypoxic Glioblastoma Cells Reveals Sequential Metabolic Adaptation of One-Carbon Metabolic Pathways. Mol Cell Proteomics, 16(11):1906-1921(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Glioblastoma,Hypoxia

Kangling Zhang
contact affiliation	Department of Pharmacology and Toxicology, School of Medicine, University of Texas Medical Branch
contact email	kazhang@utmb.edu
lab head
Kangling Zhang
contact affiliation	University of Texas Medical Branch
contact email	kazhang@utmb.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD005487

PRIDE project URI

• PRIDE
  1. PXD005487
     1. Label: PRIDE project
     2. Name: Proteome of Hypoxic Glioblastoma Cells