PXD005424 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | On tissue Top down microproteomic bridged to MALDI MS Imaging reveal the molecular physiome of brain regions |
| Description | Tissue Top-down microproteomic was performed on 3 brain regions. 156 references proteins from different cellular compartments were characterized. Moreover, 11 Alternative proteins issued from alternative open reading frames (AltORF) were identified and were relied to the brain regions and associated to the function of their reference proteins. Some proteins display specific post-translational modifications profiles or truncation linked the brain regions and their functions. Systemic biology performed on microproteome identified in each region allowed to associate sub-networks with functional physiology of each brain region. Back correlation of the local extracted and identified microproteome with tissue cellular localization was then performed by MALDI mass spectrometry imaging. 40 proteins including 4 Altproteins have been back correlated in MALDI MSI and are in line with their tissue extracted region and physiological function. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_04:33:57.395.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Julien Franck |
| SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
| ModificationList | phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2016-11-22 08:43:26 | ID requested | |
| 1 | 2017-11-14 08:25:09 | announced | |
| ⏵ 2 | 2024-10-22 04:34:05 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.1074/mcp.m116.065755; |
| Delcourt V, Franck J, Quanico J, Gimeno JP, Wisztorski M, Raffo-Romero A, Kobeissy F, Roucou X, Salzet M, Fournier I, Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions. Mol Cell Proteomics, 17(2):357-372(2018) [pubmed] |
Keyword List
| curator keyword: Biological |
| submitter keyword: Alternative Proteins,Top Down micro-proteomics, MALDI Imaging |
Contact List
| Michel Salzet |
|---|
| contact affiliation | Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM) - INSERM U1192 - Université Lille 1, Bât SN3, 1er étage, Cité Scientifique, F-59655 Villeneuve d’Ascq Cedex |
| contact email | michel.salzet@univ-lille1.fr |
| lab head | |
| Julien Franck |
|---|
| contact affiliation | PRISM-INSERM U1192 |
| contact email | julien.franck@univ-lille.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD005424 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005424
- Label: PRIDE project
- Name: On tissue Top down microproteomic bridged to MALDI MS Imaging reveal the molecular physiome of brain regions
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