PXD005404 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri |
Description | Hemolymph was characterized from Diaphorina citri adults infected with the phytopathogen, Candidatus Liberibacter asiaticus (CLas) and compared with that from uninfected psyllids. This study identified 5531 and 3220 peptides within infected and uninfected hemolymph using nano LC-MS/MS. A reduced number of proteins were detected for D. citri and all known endosymbionts within infected hemolymph as compared to uninfected hemolymph. A large number of immune defense proteins were absent from D. citri hemolymph; however, a single recognition protein (PGRP), two serine protease inhibitors, three prophenoloxidase (proPO) enzymes, and a single serine protease in an uninfected D. citri were detected. The hemolymph is nearly devoid of nutrient storage proteins. This is the first proteomic analysis of D. citri hemolymph that also analyzes the components contributed by all of the endosymbionts. By comparing the contribution of each endosymbiont (CCR, CPA,WB) in the presence and absence of CLas infection, this study provides initial insights regarding the hemolymph response to microbial community shifts associated with D. citri infection status. Our data also present potential protein targets for analysis and disruption of CLas transmission that may facilitate management of huanglongbing (HLB) caused by CLas in citrus. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-18 |
AnnouncementXML | Submission_2017-01-18_04:05:27.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD005404 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Torrence Gill |
SpeciesList | scientific name: Candidatus Profftella armatura; NCBI TaxID: 669502; scientific name: Liberibacter endosymbiont of Diaphorina citri; NCBI TaxID: 116597; scientific name: Candidatus Carsonella ruddii DC; NCBI TaxID: 667013; scientific name: Wolbachia endosymbiont of Diaphorina citri; NCBI TaxID: 116598; scientific name: Diaphorina citri (Asian citrus psyllid); NCBI TaxID: 121845; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-21 04:19:44 | ID requested | |
⏵ 1 | 2017-01-18 04:05:29 | announced | |
Publication List
Gill TA, Chu C, Pelz-Stelinski KS, Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri. Amino Acids, 49(2):389-406(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Asian Citrus Psyllid, Diaphorina citri, Endosymbionts, Hemolymph, Hemiptera |
Contact List
Kirsten Pelz-Stelinski |
contact affiliation | University of Florida Department of Entomology Citrus Research and Education Center Lake Alfred, Florida |
contact email | pelzstelinski@ufl.edu |
lab head | |
Torrence Gill |
contact affiliation | University of Florida |
contact email | gilltorrence@hotmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005404
- Label: PRIDE project
- Name: Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri