PXD005378-2

PXD005378 is an original dataset announced via ProteomeXchange.

Title	Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- whole cell lysate of HCT 116 epithelial cells, plestatins-3 competitive pull down 2h
Description	The inability of determining direct molecular targets of metal-based therapeutic agents beyond the platinum class is one of the major bottlenecks in their development. Here, we employ an integrated chemical proteomics and response profiling approach to identify and validate plestatins, based on an organometallic ruthenium(II) scaffold, as selective plectin-targeting agents. Plectin is a giant scaffold protein involved in controlling cytoarchitecture. Subcytotoxic concentrations of plestatins induce drastic morphological phenotypes on the microtubule (MT) network accompanied by a global down-regulation of translational activity. Plestatins possess anti-invasive properties in tumour spheroids and also display tumour-inhibiting effects in vivo after oral administration. The ability of plestatins to alter the MT network via plectin represents a unique mechanism to target MTs and shows promise as an anticancer strategy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:31.621.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005378
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-17 01:12:12	ID requested
1	2022-03-01 08:09:02	announced
⏵ 2	2024-10-22 05:34:38	announced	2024-10-22: Updated project metadata.

10.1002/anie.201702242;

10.6019/PXD005378;

Meier SM, Kreutz D, Winter L, Klose MHM, Cseh K, Weiss T, Bileck A, Alte B, Mader JC, Jana S, Chatterjee A, Bhattacharyya A, Hejl M, Jakupec MA, Heffeter P, Berger W, Hartinger CG, Keppler BK, Wiche G, Gerner C, An Organoruthenium Anticancer Agent Shows Unexpected Target Selectivity For Plectin. Angew Chem Int Ed Engl, 56(28):8267-8271(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Ruthenium, Response Profiling, Organometallics, Target Identification, Metallodrugs,Chemical Proteomics, Mechanism of Action, Plectin

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005378
     1. Label: PRIDE project
     2. Name: Targeting the Scaffold Protein Plectin by an Organometallic as a Strategy to Inhibit Tumour Invasiveness- whole cell lysate of HCT 116 epithelial cells, plestatins-3 competitive pull down 2h