PXD005361 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nicotine-induced protein expression profiling reveals mutually altered proteins across four human cell lines |
Description | Mass spectrometry-based proteomic strategies can profile the expression level of proteins in response to external stimuli. Nicotine affects diverse cellular pathways, however, the nicotine-induced alterations on the global proteome across human cell lines have not been fully elucidated. We measured perturbations in protein levels resulting from nicotine treatment in four cell lines - HEK, HeLa, PaSC, and SH-SY5Y- in a single experiment using tandem mass tags (TMT10-plex) and high-resolution mass spectrometry. We quantified 8590 proteins across all cell lines. Of these, nicotine increased the abundance of 31 proteins 1.5-fold or greater in all cell lines. Likewise, considering proteins with altered levels in at least 3 of the 4 cell lines, 64 were up-regulated, while 1 was down-regulated. Gene ontology analysis revealed that ~40% of these proteins were membrane-bound, and functioned in transmembrane signaling and receptor activity. We highlighted proteins, including APP, APLP2, LAPTM4B, and NCOA4, which were dysregulated by nicotine in all cell lines investigated and may have implications in downstream signaling pathways, particularly autophagy. Using the outlined methodology, studies in additional (including primary) cell lines will provide further evidence that alterations in the levels of these proteins are indeed a general response to nicotine and thereby merit further investigation. |
HostingRepository | PRIDE |
AnnounceDate | 2018-10-26 |
AnnouncementXML | Submission_2018-10-26_12:59:17.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joao Paulo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-16 02:25:07 | ID requested | |
⏵ 1 | 2018-10-26 12:59:18 | announced | |
2 | 2024-10-22 04:34:32 | announced | 2024-10-22: Updated project metadata. |
Publication List
Paulo JA, Gygi SP, Nicotine-induced protein expression profiling reveals mutually altered proteins across four human cell lines. Proteomics, 17(1-2):(2017) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: TMT, Orbitrap Fusion, nicotine, pancreas, SPS-MS3 |
Contact List
Joao A. Paulo |
contact affiliation | Harvard Medical School Cell Biology USA |
contact email | joao_paulo@post.harvard.edu |
lab head | |
Joao Paulo |
contact affiliation | Harvard Medical School |
contact email | joao_paulo@post.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005361 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005361
- Label: PRIDE project
- Name: Nicotine-induced protein expression profiling reveals mutually altered proteins across four human cell lines