PXD005323-1

PXD005323 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-Based Quantitative Proteomic Analysis of Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells
Description	Viscum coloratum (Kom.) Nakai is a variety of biological activities of medicinal plants, and the active component such as polysaccharides or alkaloids, also proved to have the effect of anti-tumor, anti-virus, anti-radiation, anti-oxidation and anti-infection. In this study, we examined the inhibition of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cells growth in a dose-dependent manner using CCK-8 kit assay, and flow cytometry analysis showed that VCP2 delayed cell cycle in G1 phase and induced apoptosis in HepG2 cells which may due to overexpressed p21Wafl/Cip1 and Cyclin D and decreased expressions of Cyclin E and CDK4, and the upregulated Bad, Smac and Caspase-3 and the downregulated Bcl-XL and XIAP may be one of reasons for inducing apoptosis in VCP2-treated HepG2 cells, but these effects may be not obviously related to Bad. Using iTRAQ and 2D-LC-MSMS, 113 differentially expressed proteins identified in normal and VCP2-treated HepG2 cells. Among them, the expressions of 59 proteins were upregulation, and the expressions of 54 proteins were downregulation. GO, pathways and PPI of differentially expressed proteins further analyzed in polysaccharides-treated HepG2 cells by bioinformatic approach. These findings widen our understanding the anti-tumor mechanisms of mistletoe polysaccharide and provide new clues for screening potent responsive protein to polysaccharides.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_13:03:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ZEPENG Yin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-11 02:35:22	ID requested
⏵ 1	2018-10-26 13:03:25	announced
2	2024-10-22 04:16:15	announced	2024-10-22: Updated project metadata.

Chai Y, Zhao M, iTRAQ-Based Quantitative Proteomic Analysis of the Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells. Sci Rep, 7(1):4596(2017) [pubmed]

curator keyword: Biological

submitter keyword: iTRAQ,HepG2,Viscum coloratum,Proteomic

Yangyang Chai
contact affiliation	Northeast forestry university
contact email	479017557@qq.com
lab head
ZEPENG Yin
contact affiliation	UF
contact email	370686292@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005323
     1. Label: PRIDE project
     2. Name: iTRAQ-Based Quantitative Proteomic Analysis of Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells