PXD005299 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific |
Description | As a well-known phenomenon, total mRNAs poorly correlate to proteins in their abundances as reported. Recent findings calculated with bivariate models suggested even poorer such correlation, whereas focusing on the translating mRNAs (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) subset. In this study, we analysed the relative abundances of mRNAs, RNC-mRNAs and proteins on genome-wide scale, comparing human lung cancer A549 and H1299 cells with normal human bronchial epithelial (HBE) cells, respectively. As discovered, a strong correlation between RNC-mRNAs and proteins in their relative abundances could be established through a multivariate linear model by integrating the mRNA length as a key factor. The R2 reached 0.94 and 0.97 in A549 versus HBE and H1299 versus HBE comparisons, respectively. This correlation highlighted that the mRNA length significantly contributes to the translational modulation, especially to the translational initiation, favoured by its correlation with the mRNA translation ratio (TR) as observed. We found TR is highly phenotype specific, which was substantiated by both pathway analysis and biased TRs of the splice variants of BDP1 gene, which is a key transcription factor of transfer RNAs. These findings revealed, for the first time, the intrinsic and genome-wide translation modulations at translatomic level in human cells at steady-state, which are tightly correlated to the protein abundance and functionally relevant to cellular phenotypes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-25 |
AnnouncementXML | Submission_2022-02-25_04:57:35.450.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yang Wang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-09 03:08:32 | ID requested | |
⏵ 1 | 2022-02-25 04:57:36 | announced | |
Publication List
Wang T, Cui Y, Jin J, Guo J, Wang G, Yin X, He QY, Zhang G, Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific. Nucleic Acids Res, 41(9):4743-54(2013) [pubmed] |
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project |
curator keyword: Biological |
submitter keyword: Translation ratios,Translating mRNAs |
Contact List
Qing-Yu He |
contact affiliation | Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and 9 Technology, Jinan University, Guangzhou 510632, China |
contact email | tqyhe@email.jnu.edu.cn |
lab head | |
Yang Wang |
contact affiliation | Jinan university |
contact email | wangyang8857@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005299
- Label: PRIDE project
- Name: Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific