PXD005257 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A |
Description | We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. To verify a knockout/knockdown of the enzymes in the insertional mutant strains, PRM measurements were performed. Mass spectrometric analyses of intact N-glycopeptides revealed that disruption of the genes altered the N-glycan composition of the alga (in single as well as double mutant). |
HostingRepository | PRIDE |
AnnounceDate | 2018-02-27 |
AnnouncementXML | Submission_2018-02-27_05:50:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stefan Schulze |
SpeciesList | scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-02 03:58:21 | ID requested | |
⏵ 1 | 2018-02-27 05:50:39 | announced | |
Publication List
Schulze S, Oltmanns A, Machnik N, Liu G, Xu N, Jarmatz N, Scholz M, Sugimoto K, Fufezan C, Huang K, Hippler M, . Plant Physiol, 176(3):1952-1964(2018) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Chlamydomonas reinhardtii, N-glycosylation, quantification |
Contact List
Michael Hippler |
contact affiliation | Institute of Plant Biology and Biotechnology, University of Muenster |
contact email | mhippler@uni-muenster.de |
lab head | |
Stefan Schulze |
contact affiliation | University of Muenster |
contact email | stefan.schulze@uni-muenster.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005257
- Label: PRIDE project
- Name: Chlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A