PXD005254-1

PXD005254 is an original dataset announced via ProteomeXchange.

Title	Chlamydomonas reinhardtii Mannosidase 1A and Xylosyltransferase1A N-glycosylation LC/MS-MS
Description	We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. Mass spectrometric analyses of intact N-glycopeptides revealed that disruption of the genes altered the N-glycan composition of the alga (in single as well as double mutant). In contrast to wildtype, methylated hexoses and terminal xylose were nearly absent in N-glycopeptides from the Man1A mutant strain whereas the XylT1A mutant showed a lack of core xylose and excessively trimmed N-glycans. The double mutant did not show this excessive trimming, thus Man1A-dependent trimming can be concluded in C. reinhardtii. Furthermore, these data indicate that methylation of hexoses is tightly interlinked with Man1A function, pointing to an enzymatic cascade in the N-glycosylation pathway.
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_05:37:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-02 03:01:31	ID requested
⏵ 1	2018-02-27 05:37:20	announced

Schulze S, Oltmanns A, Machnik N, Liu G, Xu N, Jarmatz N, Scholz M, Sugimoto K, Fufezan C, Huang K, Hippler M, . Plant Physiol, 176(3):1952-1964(2018) [pubmed]

curator keyword: Biological

submitter keyword: Chlamydomonas reinhardtii, N-gylcoproteomics, LC-MS/MS

Michael Hippler
contact affiliation	Institute of Plant Biology and Biotechnology, University of Muenster
contact email	mhippler@uni-muenster.de
lab head
Stefan Schulze
contact affiliation	University of Muenster
contact email	stefan.schulze@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005254
     1. Label: PRIDE project
     2. Name: Chlamydomonas reinhardtii Mannosidase 1A and Xylosyltransferase1A N-glycosylation LC/MS-MS