PXD005228-1

PXD005228 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic Analysis of Hair Shafts from Monozygotic Twins: Expression Profiles and Genetically Variable Peptides
Description	Forensic association of hair shaft evidence with individuals is currently assessed by comparing mitochondrial DNA haplotypes of reference and casework samples, primarily for exclusionary purposes. Present work tests and validates more recent proteomic approaches to extract quantitative transcriptional and genetic information from hair samples of monozygotic twin pairs, which would be predicted to partition away from unrelated individuals if the datasets contain identifying information. Protein expression profiles and polymorphic, genetically variant hair peptides were generated from 10 pairs of monozygotic twins. Profiling using the protein tryptic digests revealed that samples from identical twins were considerably more alike in profile than unrelated individuals among the twins. The data did not indicate that the degree of difference within twin pairs increased with age. In parallel, data from the digests were used to detect genetically variant peptides that result from common non-synonymous single nucleotide polymorphisms in genes expressed in the hair follicle. Compilation of the variants permitted sorting of the samples by hierarchical clustering, permitting accurate matching of twin pairs. The results demonstrate that genetic differences are detectable by proteomic methods and provide a framework for developing quantitative statistical estimates of personal identification that increase the value of hair shaft evidence.
HostingRepository	MassIVE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_09:12:36.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brett Phinney
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Deamidated; Carboxymethyl
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-10-26 11:01:30	ID requested
⏵ 1	2018-12-04 09:12:36	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: hair

submitter keyword: hair

Contact List

Robert H. Rice
lab head
Brett Phinney
contact affiliation	UC Davis
contact email	brettsp@ucdavis.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080295